Source:http://linkedlifedata.com/resource/pubmed/id/11517608
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2001-8-23
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pubmed:abstractText |
To study the position and immunogenicity of rSP10, the rabbit rsp10 gene which did not include sequences coding for signal peptides was inserted into expression vector pET30a. An in-frame fusion protein was made such that a His6 stretch was produced at the N terminus of re-rSP10. High expression was obtained, the amount of re-rSP10 up to 67% in the total bacterial protein. The re-rSP10 was purified by DEAE chromatography and the yield of purified re-rSP10 was approximately 50 micrograms/mL of culture. Western blotting analysis of re-rSP10 with rabbit polyclonal sera raised against rabbit sperm membrane protein showed that the synthesized antigen possessed immunogenicity of rSP10. Specific antisera against re-rSP10 was induced using purified re-rSP10 as an antigen. The motility of capacitated sperms were affected but no aggregation was observed.
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pubmed:language |
chi
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rSMP-B protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1000-3061
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
314-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11517608-Animals,
pubmed-meshheading:11517608-Antigens, Surface,
pubmed-meshheading:11517608-Escherichia coli,
pubmed-meshheading:11517608-Female,
pubmed-meshheading:11517608-Immune Sera,
pubmed-meshheading:11517608-Male,
pubmed-meshheading:11517608-Membrane Proteins,
pubmed-meshheading:11517608-Plasmids,
pubmed-meshheading:11517608-Rabbits,
pubmed-meshheading:11517608-Recombinant Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
[High expression of rabbit sperm membrane protein rSP10 in Escherichia coli and preparation of its specific antisera].
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pubmed:affiliation |
National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
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pubmed:publicationType |
Journal Article,
English Abstract
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