Linked Life Data

11517386

Source:http://linkedlifedata.com/resource/pubmed/id/11517386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-8-22
pubmed:abstractText
Glial cells function as sensors for infection within the brain and produce cytokines to limit viral replication and spread. We examined both cytokine (TNF-alpha, IL-1beta, and IL-6) and chemokine (MCP-1, MIP-1alpha, RANTES, and IL-8) production by primary human glial cells in response to cytomegalovirus (CMV). Although CMV-infected astrocytes did not produce antiviral cytokines, they generated significant quantities of the chemokines MCP-1 and IL-8 in response to viral infection. On the other hand, supernatants from CMV-stimulated purified microglial cell cultures showed a marked increase in the production of TNF-alpha and IL-6, as well as chemokines. Supernatants from CMV-infected astrocyte cultures induced the migration of microglia towards chemotactic signals generated from infected astrocytes. Antibodies to MCP-1, but not to MIP-1alpha, RANTES, or IL-8, inhibited this migratory activity. These findings suggest that infected astrocytes may use MCP-1 to recruit antiviral cytokine-producing microglial cells to foci of infection. To test this hypothesis, cocultures of astrocytes and microglial cells were infected with CMV. Viral gene expression in these cocultures was 60% lower than in CMV infected purified astrocyte cultures lacking microglia. These results support the hypothesis that microglia play an important antiviral role in defense of the brain against CMV. The host defense function of microglial cells may be directed in part by chemokines, such as MCP-1, produced by infected astrocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1355-0284
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-47
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11517386-Astrocytes, pubmed-meshheading:11517386-Brain, pubmed-meshheading:11517386-Cells, Cultured, pubmed-meshheading:11517386-Chemokine CCL3, pubmed-meshheading:11517386-Chemokine CCL4, pubmed-meshheading:11517386-Chemokine CCL5, pubmed-meshheading:11517386-Chemotaxis, pubmed-meshheading:11517386-Coculture Techniques, pubmed-meshheading:11517386-Cytomegalovirus, pubmed-meshheading:11517386-Cytomegalovirus Infections, pubmed-meshheading:11517386-Encephalitis, Viral, pubmed-meshheading:11517386-Fetus, pubmed-meshheading:11517386-Gene Expression Regulation, Viral, pubmed-meshheading:11517386-Humans, pubmed-meshheading:11517386-Interleukin-1, pubmed-meshheading:11517386-Interleukin-6, pubmed-meshheading:11517386-Interleukin-8, pubmed-meshheading:11517386-Macrophage Inflammatory Proteins, pubmed-meshheading:11517386-Microglia, pubmed-meshheading:11517386-RNA, Messenger, pubmed-meshheading:11517386-Tumor Necrosis Factor-alpha, pubmed-meshheading:11517386-Virus Replication
pubmed:year
2001
pubmed:articleTitle
Cytomegalovirus induces cytokine and chemokine production differentially in microglia and astrocytes: antiviral implications.
pubmed:affiliation
Institute for Brain and Immune Disorders, Minneapolis Medical Research Foundation, Minneapolis, Minnesota 55404, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.