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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
42
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pubmed:dateCreated |
2001-10-15
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pubmed:abstractText |
Cytosolic Ca(2+) oscillations can be due to cycles of release and re-uptake of internally stored Ca(2+). To investigate the nature of these Ca(2+) stores, we expressed the Pmr1 Ca(2+) pump of Caenorhabditis elegans in COS-1 cells and pretreated the cells with thapsigargin to prevent Ca(2+) uptake by the sarco(endo)plasmic reticulum Ca(2+)-ATPase. Pmr1 co-localized with the Golgi-specific 58K protein and was targeted to a Ca(2+) store that was less leaky for Ca(2+) than the endoplasmic reticulum and whose inositol trisphosphate receptors were less sensitive to inositol trisphosphate and ATP than those in the endoplasmic reticulum. ATP-stimulated Pmr1-overexpressing cells responded after a latency to extracellular Ca(2+) with a regenerative Ca(2+) signal, which could be prevented by caffeine. They also produced very stable ilimaquinone-sensitive baseline Ca(2+) spikes, even in the presence of thapsigargin. Such responses never occurred in non-transfected cells or in cells that overexpressed the type-1 sarco(endo)plasmic reticulum Ca(2+)-ATPase. Abortive Ca(2+) spikes also occurred in histamine-stimulated untransfected HeLa cells pretreated with thapsigargin, and they too were inhibited by ilimaquinone. We conclude that the Pmr1-induced Ca(2+) store, which probably corresponds to the Golgi compartment, can play a crucial role in setting up baseline Ca(2+) spiking.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Caffeine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Central Nervous System Stimulants,
http://linkedlifedata.com/resource/pubmed/chemical/ITPR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:CallewaertGG,
pubmed-author:De SmedtHH,
pubmed-author:MissiaenLL,
pubmed-author:ParysJ BJB,
pubmed-author:RaeymaekersLL,
pubmed-author:RendersJJ,
pubmed-author:RizzutoRR,
pubmed-author:Van AckerKK,
pubmed-author:Van BaelenKK,
pubmed-author:VanoevelenJJ,
pubmed-author:WeidemaA FAF,
pubmed-author:WuytackFF
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39161-70
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pubmed:dateRevised |
2007-7-18
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pubmed:meshHeading |
pubmed-meshheading:11514551-Adenosine Triphosphate,
pubmed-meshheading:11514551-Animals,
pubmed-meshheading:11514551-COS Cells,
pubmed-meshheading:11514551-Caenorhabditis elegans,
pubmed-meshheading:11514551-Caffeine,
pubmed-meshheading:11514551-Calcium,
pubmed-meshheading:11514551-Calcium Channels,
pubmed-meshheading:11514551-Cell Line,
pubmed-meshheading:11514551-Central Nervous System Stimulants,
pubmed-meshheading:11514551-Cytosol,
pubmed-meshheading:11514551-Endoplasmic Reticulum,
pubmed-meshheading:11514551-Golgi Apparatus,
pubmed-meshheading:11514551-HeLa Cells,
pubmed-meshheading:11514551-Humans,
pubmed-meshheading:11514551-Immunoblotting,
pubmed-meshheading:11514551-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:11514551-Microscopy, Fluorescence,
pubmed-meshheading:11514551-Protein Binding,
pubmed-meshheading:11514551-Rabbits,
pubmed-meshheading:11514551-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:11514551-Signal Transduction,
pubmed-meshheading:11514551-Time Factors,
pubmed-meshheading:11514551-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store.
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pubmed:affiliation |
Laboratorium voor Fysiologie, K.U. Leuven Campus Gasthuisberg O/N, Herestraat 49, Leuven B-3000, Belgium. Ludwig.Missiaen@med.kuleuven.ac.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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