Linked Life Data

11497102

Source:http://linkedlifedata.com/resource/pubmed/id/11497102

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SubjectPredicateObjectContext
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pubmed-article:11497102pubmed:dateCreated2001-8-10lld:pubmed
pubmed-article:11497102pubmed:abstractTextThe photoinduced currents in whole chloroplasts of Peperomia metallica were studied using suction electrodes and single-turnover flashes. The kinetic profile of the photocurrent contained a minor outward component (rise time, 100 micros). Local application (from the inside of the pipette) of a photosystem 2 inhibitor, DCMU, rapidly suppressed the outward current; conversely, addition of DCMU to the outer medium produced a transient stimulation of the outward component. Permeabilization of the tip-located membrane fragments with Triton X-100 eliminated the outward current, but had no significant influence on the inward current. The data suggest that the outward current originated in the tip-located nonruptured portions of the thylakoid membrane. Different involvement of two photosystems in the generation of the outward current indicates that granal thylakoids enriched with photosystem 2 are less susceptible to the rupture in the pipette tip as compared with stromal thylakoids.lld:pubmed
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pubmed-article:11497102pubmed:pagination475-85lld:pubmed
pubmed-article:11497102pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11497102pubmed:year2001lld:pubmed
pubmed-article:11497102pubmed:articleTitleThe outward component of photoinduced current in chloroplasts of Peperomia metallica.lld:pubmed
pubmed-article:11497102pubmed:affiliationLomonosov Moscow State University, Faculty of Biology, Russia. cherkashin@biophys.msu.rulld:pubmed
pubmed-article:11497102pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11497102pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed