Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-8-10
pubmed:abstractText
The photoinduced currents in whole chloroplasts of Peperomia metallica were studied using suction electrodes and single-turnover flashes. The kinetic profile of the photocurrent contained a minor outward component (rise time, 100 micros). Local application (from the inside of the pipette) of a photosystem 2 inhibitor, DCMU, rapidly suppressed the outward current; conversely, addition of DCMU to the outer medium produced a transient stimulation of the outward component. Permeabilization of the tip-located membrane fragments with Triton X-100 eliminated the outward current, but had no significant influence on the inward current. The data suggest that the outward current originated in the tip-located nonruptured portions of the thylakoid membrane. Different involvement of two photosystems in the generation of the outward current indicates that granal thylakoids enriched with photosystem 2 are less susceptible to the rupture in the pipette tip as compared with stromal thylakoids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1023-6597
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
475-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
The outward component of photoinduced current in chloroplasts of Peperomia metallica.
pubmed:affiliation
Lomonosov Moscow State University, Faculty of Biology, Russia. cherkashin@biophys.msu.ru
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't