rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-8-10
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pubmed:abstractText |
The photoinduced currents in whole chloroplasts of Peperomia metallica were studied using suction electrodes and single-turnover flashes. The kinetic profile of the photocurrent contained a minor outward component (rise time, 100 micros). Local application (from the inside of the pipette) of a photosystem 2 inhibitor, DCMU, rapidly suppressed the outward current; conversely, addition of DCMU to the outer medium produced a transient stimulation of the outward component. Permeabilization of the tip-located membrane fragments with Triton X-100 eliminated the outward current, but had no significant influence on the inward current. The data suggest that the outward current originated in the tip-located nonruptured portions of the thylakoid membrane. Different involvement of two photosystems in the generation of the outward current indicates that granal thylakoids enriched with photosystem 2 are less susceptible to the rupture in the pipette tip as compared with stromal thylakoids.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-phenylenediamine,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/Diuron,
http://linkedlifedata.com/resource/pubmed/chemical/Gramicidin,
http://linkedlifedata.com/resource/pubmed/chemical/Herbicides,
http://linkedlifedata.com/resource/pubmed/chemical/Methylphenazonium Methosulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Octoxynol,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylenediamines,
http://linkedlifedata.com/resource/pubmed/chemical/Photosynthetic Reaction Center...
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pubmed:status |
MEDLINE
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pubmed:issn |
1023-6597
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-85
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11497102-Anti-Bacterial Agents,
pubmed-meshheading:11497102-Chloroplasts,
pubmed-meshheading:11497102-Coloring Agents,
pubmed-meshheading:11497102-Detergents,
pubmed-meshheading:11497102-Diuron,
pubmed-meshheading:11497102-Energy Transfer,
pubmed-meshheading:11497102-Gramicidin,
pubmed-meshheading:11497102-Herbicides,
pubmed-meshheading:11497102-Light,
pubmed-meshheading:11497102-Membrane Potentials,
pubmed-meshheading:11497102-Methylphenazonium Methosulfate,
pubmed-meshheading:11497102-Octoxynol,
pubmed-meshheading:11497102-Patch-Clamp Techniques,
pubmed-meshheading:11497102-Phenylenediamines,
pubmed-meshheading:11497102-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:11497102-Piperaceae,
pubmed-meshheading:11497102-Plant Leaves
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pubmed:year |
2001
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pubmed:articleTitle |
The outward component of photoinduced current in chloroplasts of Peperomia metallica.
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pubmed:affiliation |
Lomonosov Moscow State University, Faculty of Biology, Russia. cherkashin@biophys.msu.ru
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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