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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
2001-8-7
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pubmed:abstractText |
Cellulose-binding modules (CBMs) of two extracellular matrix proteins, St15 and ShD, from the slime mold Dictyostelium discoideum were expressed in Escherichia coli. The expressed proteins were purified to > 98% purity by extracting inclusion bodies at pH 11.5 and refolding proteins at pH 7.5. The two refolded CBMs bound tightly to amorphous phosphoric acid swollen cellulose (PASC), but had a low affinity toward xylan. Neither protein exhibited cellulase activity. St15, the stalk-specific protein, had fourfold higher binding affinity toward microcrystalline cellulose (Avicel) than the sheath-specific ShD CBM. St15 is unusual in that it consists of a solitary CBM homologous to family IIa CBMs. Sequence analysis of ShD reveals three putative domains containing: (a) a C-terminal CBM homologous to family IIb CBMs; (b) a Pro/Thr-rich linker domain; and (c) a N-terminal Cys-rich domain. The biological functions and potential role of St15 and ShD in building extracellular matrices during D. discoideum development are discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cellulase,
http://linkedlifedata.com/resource/pubmed/chemical/Cellulose,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Coomassie blue,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rosaniline Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Salts
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4334-45
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:11488929-Amino Acid Sequence,
pubmed-meshheading:11488929-Animals,
pubmed-meshheading:11488929-Base Sequence,
pubmed-meshheading:11488929-Binding Sites,
pubmed-meshheading:11488929-Blotting, Western,
pubmed-meshheading:11488929-Cellulase,
pubmed-meshheading:11488929-Cellulose,
pubmed-meshheading:11488929-Cloning, Molecular,
pubmed-meshheading:11488929-Coloring Agents,
pubmed-meshheading:11488929-DNA, Complementary,
pubmed-meshheading:11488929-Dictyostelium,
pubmed-meshheading:11488929-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11488929-Escherichia coli,
pubmed-meshheading:11488929-Extracellular Matrix,
pubmed-meshheading:11488929-Hydrogen-Ion Concentration,
pubmed-meshheading:11488929-Kinetics,
pubmed-meshheading:11488929-Models, Biological,
pubmed-meshheading:11488929-Molecular Sequence Data,
pubmed-meshheading:11488929-Polymerase Chain Reaction,
pubmed-meshheading:11488929-Protein Binding,
pubmed-meshheading:11488929-Protein Folding,
pubmed-meshheading:11488929-Protein Structure, Tertiary,
pubmed-meshheading:11488929-Proto-Oncogene Proteins,
pubmed-meshheading:11488929-Protozoan Proteins,
pubmed-meshheading:11488929-Recombinant Fusion Proteins,
pubmed-meshheading:11488929-Recombinant Proteins,
pubmed-meshheading:11488929-Rosaniline Dyes,
pubmed-meshheading:11488929-Salts,
pubmed-meshheading:11488929-Sequence Analysis, Protein,
pubmed-meshheading:11488929-Sequence Homology, Amino Acid
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pubmed:year |
2001
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pubmed:articleTitle |
Cellulose-binding modules from extracellular matrix proteins of Dictyostelium discoideum stalk and sheath.
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pubmed:affiliation |
Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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