Source:http://linkedlifedata.com/resource/pubmed/id/11483222
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2001-8-2
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| pubmed:abstractText |
A chimeric cDNA genome was constructed in which the core, E1 and E2 genes of hepatitis C virus (HCV) replaced the core, E(rns), E1 and E2 genes of bovine viral diarrhea virus (BVDV). High levels of HCV structural proteins were expressed in a small number of human or bovine cells following transfection with chimeric RNA. However, in one cell line, bovine embryonic trachea cells [EBTr(A)], the number of cells expressing HCV proteins increased to greater than 70% following serial passage of culture medium. These cells were persistently infected with a non-cytopathogenic BVDV helper virus. In these cells, the chimeric genome was packaged into infectious particles that accumulated in the culture medium at a titer as high as 10(7)-10(9) genome equivalents per ml. The virus particles were pseudotypes, because they were neutralized by anti-BVDV but not by anti-HCV.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0166-0934
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
97
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
113-23
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11483222-Animals,
pubmed-meshheading:11483222-Antibodies, Viral,
pubmed-meshheading:11483222-Blotting, Western,
pubmed-meshheading:11483222-Cattle,
pubmed-meshheading:11483222-DNA, Recombinant,
pubmed-meshheading:11483222-Diarrhea Viruses, Bovine Viral,
pubmed-meshheading:11483222-Gene Expression Regulation, Viral,
pubmed-meshheading:11483222-Genetic Engineering,
pubmed-meshheading:11483222-Genome, Viral,
pubmed-meshheading:11483222-Helper Viruses,
pubmed-meshheading:11483222-Hepacivirus,
pubmed-meshheading:11483222-Humans,
pubmed-meshheading:11483222-Immune Sera,
pubmed-meshheading:11483222-Microscopy, Fluorescence,
pubmed-meshheading:11483222-Radioimmunoprecipitation Assay,
pubmed-meshheading:11483222-Transfection,
pubmed-meshheading:11483222-Tumor Cells, Cultured,
pubmed-meshheading:11483222-Viral Structural Proteins,
pubmed-meshheading:11483222-Virus Replication
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| pubmed:year |
2001
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| pubmed:articleTitle |
High-level expression of hepatitis C virus (HCV) structural proteins by a chimeric HCV/BVDV genome propagated as a BVDV pseudotype.
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| pubmed:affiliation |
Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0740, USA.
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| pubmed:publicationType |
Journal Article
|