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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5530
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pubmed:dateCreated |
2001-7-27
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pubmed:databankReference |
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pubmed:abstractText |
The dynamics and polarity of actin filaments are controlled by a conformational change coupled to the hydrolysis of adenosine 5'-triphosphate (ATP) by a mechanism that remains to be elucidated. Actin modified to block polymerization was crystallized in the adenosine 5'-diphosphate (ADP) state, and the structure was solved to 1.54 angstrom resolution. Compared with previous ATP-actin structures from complexes with deoxyribonuclease I, profilin, and gelsolin, monomeric ADP-actin is characterized by a marked conformational change in subdomain 2. The successful crystallization of monomeric actin opens the way to future structure determinations of actin complexes with actin-binding proteins such as myosin.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
293
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
708-11
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11474115-Actins,
pubmed-meshheading:11474115-Adenosine Diphosphate,
pubmed-meshheading:11474115-Adenosine Triphosphate,
pubmed-meshheading:11474115-Binding Sites,
pubmed-meshheading:11474115-Biopolymers,
pubmed-meshheading:11474115-Calcium,
pubmed-meshheading:11474115-Crystallization,
pubmed-meshheading:11474115-Crystallography, X-Ray,
pubmed-meshheading:11474115-Deoxyribonuclease I,
pubmed-meshheading:11474115-Hydrogen Bonding,
pubmed-meshheading:11474115-Models, Molecular,
pubmed-meshheading:11474115-Phosphates,
pubmed-meshheading:11474115-Protein Conformation,
pubmed-meshheading:11474115-Protein Folding,
pubmed-meshheading:11474115-Protein Structure, Secondary,
pubmed-meshheading:11474115-Protein Structure, Tertiary,
pubmed-meshheading:11474115-Rhodamines
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pubmed:year |
2001
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pubmed:articleTitle |
The crystal structure of uncomplexed actin in the ADP state.
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pubmed:affiliation |
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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