Linked Life Data

11472922

Source:http://linkedlifedata.com/resource/pubmed/id/11472922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-7-26
pubmed:abstractText
Bacteria of numerous species isolated from the human gastrointestinal tract express bile salt hydrolase (BSH) activity. How this activity contributes to functions of the microorganisms in the gastrointestinal tract is not known. We tested the hypothesis that a BSH protects the cells that produce it from the toxicity of conjugated bile salts. Forty-nine strains of numerous Lactobacillus spp. were assayed to determine their capacities to express BSH activities (taurodeoxycholic acid [TDCA] hydrolase and taurocholic acid [TCA] hydrolase activities) and their capacities to resist the toxicity of a conjugated bile acid (TDCA). Thirty of these strains had been isolated from the human intestine, 15 had been recovered from dairy products, and 4 had originated from other sources. Twenty-six of the strains expressed both TDCA hydrolase and TCA hydrolase activities. One strain that expressed TDCA hydrolase activity did not express TCA hydrolase activity. Conversely, in one strain for which the assay for TDCA hydrolase activity gave a negative result there was evidence of TCA hydrolase activity. Twenty-five of the strains were found to resist the toxicity of TDCA. Fourteen of these strains were of human origin, nine were from dairy products, and two were from other sources. Of the 26 strains expressing both TDCA hydrolase and TCA hydrolase activities, 15 were resistant to TDCA toxicity, 6 were susceptible, and 5 gave inconclusive results. Of the 17 strains that gave negative results for either of the enzymes, 7 were resistant to the toxicity, 9 were susceptible, and 1 gave inconclusive results. These findings do not support the hypothesis tested. They suggest, however, that BSH activity is important at some level for lactobacillus colonization of the human intestine.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-10543808, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-13710, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-1622282, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-1987119, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-2376558, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-2527484, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-2705765, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-2876046, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-3178214, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-7055372, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-7592123, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-7811085, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-8779581, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-9603834, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-9721268, http://linkedlifedata.com/resource/pubmed/commentcorrection/11472922-9872808
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3476-80
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Bile salt hydrolase activity and resistance to toxicity of conjugated bile salts are unrelated properties in lactobacilli.
pubmed:affiliation
Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't