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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-7-26
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pubmed:abstractText |
CVI is a primary immunodeficiency characterized by a failure of B cell differentiation associated with an array of T cell defects, such as enhanced T cell apoptosis. In this study we investigated the mechanisms underlying CVI enhanced T cell death. We analysed both the expression of Fas using flow cytometry techniques and the expression of FasL mRNA using RT-PCR in CVI T cells. We could not find any significant differences between CVI and normal subjects with regard to Fas expression, although there was a subgroup of CVI patients with very high Fas expression which was accompanied by an up-regulation of FasL mRNA. However, attempts to induce Fas-mediated apoptosis in these high Fas expressing cells, as evaluated by propidium iodide staining and APO2.7 staining, were unsuccessful. We also investigated intracellular levels of Bcl-2, bcl-xl and bax in CD4(+) and CD8(+) CVI T cells, as well as the bax/Bcl-2 ratio, using flow cytometry techniques but could not detect any differences between CVI and normal subjects. Finally we analysed TNF-RI and TNF-RII mRNA expression in CD4(+) and CD8(+) CVI T cells using semiquantitative RT-PCR and found a significant increase in expression of both TNF-Rs in CD4(+) T cells from CVI patients. Our data suggest that the increased expression of both TNF-Rs on T cells may be one of the mechanisms responsible for the accelerated T cell apoptosis in CVI.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-10403927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-10413651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-10415029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-10844530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-1371242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-1395097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-2110212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-2472237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-2515013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7533326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7533498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7539010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7566090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7632946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7634826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-7906214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8431353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8566072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8579749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8617294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8621473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8625402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8825415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-8910673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-9367420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-9415413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-9557163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11472434-9973490
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/BCL2L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11472434-Adolescent,
pubmed-meshheading:11472434-Adult,
pubmed-meshheading:11472434-Aged,
pubmed-meshheading:11472434-Antigens, CD,
pubmed-meshheading:11472434-Antigens, CD95,
pubmed-meshheading:11472434-Apoptosis,
pubmed-meshheading:11472434-Child,
pubmed-meshheading:11472434-Common Variable Immunodeficiency,
pubmed-meshheading:11472434-Fas Ligand Protein,
pubmed-meshheading:11472434-Gene Expression,
pubmed-meshheading:11472434-Humans,
pubmed-meshheading:11472434-Membrane Glycoproteins,
pubmed-meshheading:11472434-Middle Aged,
pubmed-meshheading:11472434-Proto-Oncogene Proteins,
pubmed-meshheading:11472434-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11472434-RNA, Messenger,
pubmed-meshheading:11472434-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:11472434-Receptors, Tumor Necrosis Factor, Type I,
pubmed-meshheading:11472434-Receptors, Tumor Necrosis Factor, Type II,
pubmed-meshheading:11472434-T-Lymphocytes,
pubmed-meshheading:11472434-bcl-2-Associated X Protein,
pubmed-meshheading:11472434-bcl-X Protein
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pubmed:year |
2001
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pubmed:articleTitle |
Enhanced T cell apoptosis in common variable immunodeficiency: negative role of the fas/fasligand system and of the Bcl-2 family proteins and possible role of TNF-RS.
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pubmed:affiliation |
Division of Clinical Immunology, The Mount Sinai Medical Center, 1425 Madison Avenue, New York City, New York, USA. cd28apo@hotmail.com
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pubmed:publicationType |
Journal Article
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