Linked Life Data

11471215

Source:http://linkedlifedata.com/resource/pubmed/id/11471215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-7-26
pubmed:abstractText
The present study was undertaken to investigate the effects of ceramide on progesterone production and apoptosis in rat luteal cells in vitro. Luteal cells were prepared from the ovaries of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) primed female Wistar rats and incubated with cell permeable C2-ceramide. The effects of ceramide on progesterone production and apoptosis in luteal cells were assessed by radio immunoassay (RIA) and flow cytometry analysis. In addition, changes in nitric oxide synthase (NOS) activity and nitric oxide (NO) production by luteal cells treated with C2-ceramide were also evaluated. Ceramide was found to reduce hCG-stimulated progesterone production in a dose-dependent manner, whereas it had little effect on basal progesterone content. Spontaneous apoptosis in luteal cells was observed after a 12-hour incubation in vitro and 5 mumol/L of ceramide significantly increased the apoptotic rate (P < 0.05). Enhanced apoptotic peak was seen in the histogram by flow cytometry. Moreover, 50 mumol/L of C2-ceramide significantly increased NOS activity (P < 0.01) and NO production (P < 0.001). It is suggested that ceramide may serve as an important signaling molecule mediating certain ovarian processes, such as luteal regression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0371-0874
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
142-6
pubmed:dateRevised
2006-4-3
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Inhibition of steroidogenesis and induction of apoptosis in rat luteal cells by cell-permeable ceramide in vitro.
pubmed:affiliation
Laboratory of Reproductive Endocrinology, Department of Physiology, Harbin Medical University, Harbin 150086. liql@ems.hrbmu.edu.cn
pubmed:publicationType
Journal Article