Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2001-7-11
pubmed:abstractText
Ribosomal precursor particles are assembled in the nucleolus before export into the cytoplasm. Using a visual assay for nuclear accumulation of 60S subunits, we have isolated several conditional-lethal strains with defects in ribosomal export (rix mutants). Here we report the characterization of a mutation in an essential gene, RIX7, which encodes a novel member of the AAA ATPase superfamily. The rix7-1 temperature-sensitive allele carries a point mutation that causes defects in pre-rRNA processing, biogenesis of 60S ribosomal subunits, and their subsequent export into the cytoplasm. Rix7p, which associates with 60S ribosomal precursor particles, localizes throughout the nucleus in exponentially growing cells, but concentrates in the nucleolus in stationary phase cells. When cells resume growth upon shift to fresh medium, Rix7p-green fluorescent protein exhibits a transient perinuclear location. We propose that a nuclear AAA ATPase is required for restructuring nucleoplasmic 60S pre-ribosomal particles to make them competent for nuclear export.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10385518, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10567516, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10588711, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10656814, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10684247, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10690410, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-10893252, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-11071906, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-11086007, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-11163219, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-11163220, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-1122947, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-11313466, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-11371346, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-1372441, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-1628825, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-1763709, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-3327689, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-8431947, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-8565072, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9159086, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9214641, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9271391, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9286697, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9323132, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9372940, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9717241, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9732280, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9774653, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9774696, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9927482, http://linkedlifedata.com/resource/pubmed/commentcorrection/11447111-9971735
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3695-704
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11447111-Adenosine Triphosphatases, pubmed-meshheading:11447111-Base Sequence, pubmed-meshheading:11447111-Biological Transport, pubmed-meshheading:11447111-Cell Nucleolus, pubmed-meshheading:11447111-Cell Nucleus, pubmed-meshheading:11447111-Cytoplasm, pubmed-meshheading:11447111-DNA Primers, pubmed-meshheading:11447111-Green Fluorescent Proteins, pubmed-meshheading:11447111-Luminescent Proteins, pubmed-meshheading:11447111-Mutation, pubmed-meshheading:11447111-Nuclear Proteins, pubmed-meshheading:11447111-RNA, Ribosomal, pubmed-meshheading:11447111-RNA Processing, Post-Transcriptional, pubmed-meshheading:11447111-Recombinant Fusion Proteins, pubmed-meshheading:11447111-Ribosomes, pubmed-meshheading:11447111-Saccharomyces cerevisiae, pubmed-meshheading:11447111-Saccharomyces cerevisiae Proteins
pubmed:year
2001
pubmed:articleTitle
A nuclear AAA-type ATPase (Rix7p) is required for biogenesis and nuclear export of 60S ribosomal subunits.
pubmed:affiliation
BZH, Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't