rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
2001-7-10
|
pubmed:abstractText |
Transcription of laccase and cellulase genes of Lentinula edodes was examined during growth and development under different temperature and moisture levels on a sawdust-based substrate. RNA was extracted from samples of mycelium and fruit bodies at various stages of development and gene expression was determined by competitive RT-PCR. The level of laccase transcripts was maximal during the mycelial growth stage, and then declined rapidly at the fruiting stage. In contrast, the cellulase transcript level peaked at the veil-break stage during fruit body development. Gene expressions for laccase and cellulase were triggered by microclimatic changes, specifically lower temperature and osmotic pressure.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0378-1097
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
10
|
pubmed:volume |
201
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
111-5
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11445176-Cellulase,
pubmed-meshheading:11445176-Culture Media,
pubmed-meshheading:11445176-Gene Expression Regulation, Fungal,
pubmed-meshheading:11445176-Laccase,
pubmed-meshheading:11445176-Lentinula,
pubmed-meshheading:11445176-Osmotic Pressure,
pubmed-meshheading:11445176-Oxidoreductases,
pubmed-meshheading:11445176-RNA, Fungal,
pubmed-meshheading:11445176-RNA, Messenger,
pubmed-meshheading:11445176-Temperature,
pubmed-meshheading:11445176-Transcription, Genetic,
pubmed-meshheading:11445176-Wood
|
pubmed:year |
2001
|
pubmed:articleTitle |
Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust.
|
pubmed:affiliation |
Department of Forest and Forest Products Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. ohgasfor@mbox.nc.kyushu-u.ac.jp
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|