Linked Life Data

11441057

Source:http://linkedlifedata.com/resource/pubmed/id/11441057

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 12
pubmed:dateCreated
2001-7-6
pubmed:abstractText
Embryos and larvae of the African catfish Clarias gariepinus excrete significant quantities of urea. The present study focused on the potential urea-generating pathways during early development of this teleost; uricolysis, argininolysis and the ornithine-urea cycle (OUC). Uricase, allantoinase, allantoicase and ureidoglycollate lyase of the uricolytic pathway were expressed in all early life stages and in adult liver of C. gariepinus. Uricase activity increased in starved larvae compared with yolk-sac larvae. The key regulatory enzyme of the teleost OUC, carbamoyl phosphate synthetase III (CPSase III), was expressed predominantly in muscle of developing C. gariepinus larvae and showed negligible activity in the absence of its allosteric effector N-acetyl-L-glutamate. CPSase III and ornithine carbamoyl transferase activities increased in fed larvae compared with starved larvae. In contrast to the early developmental stages, adult C. gariepinus expressed only low and variable levels of CPSase III, suggesting that, under the experimental conditions employed, OUC expression is influenced by developmental stage in this species. The data indicate that early C. gariepinus life stages express the enzymes necessary for urea production by uricolysis, argininolysis and the OUC, and this may explain why urea tissue levels and urea excretion rates are substantial during the early development of this air-breathing teleost.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Amidine-Lyases, http://linkedlifedata.com/resource/pubmed/chemical/Amidohydrolases, http://linkedlifedata.com/resource/pubmed/chemical/Arginase, http://linkedlifedata.com/resource/pubmed/chemical/Carbamoyl-Phosphate Synthase..., http://linkedlifedata.com/resource/pubmed/chemical/Carbon-Nitrogen Ligases, http://linkedlifedata.com/resource/pubmed/chemical/Glutamate-Ammonia Ligase, http://linkedlifedata.com/resource/pubmed/chemical/Urate Oxidase, http://linkedlifedata.com/resource/pubmed/chemical/Urea, http://linkedlifedata.com/resource/pubmed/chemical/Ureohydrolases, http://linkedlifedata.com/resource/pubmed/chemical/allantoicase, http://linkedlifedata.com/resource/pubmed/chemical/allantoinase, http://linkedlifedata.com/resource/pubmed/chemical/carbamoyl-phosphate synthetase..., http://linkedlifedata.com/resource/pubmed/chemical/ureidoglycollate lyase
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-0949
pubmed:author
pubmed:issnType
Print
pubmed:volume
204
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2155-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Pathways for urea production during early life of an air-breathing teleost, the African catfish Clarias gariepinus Burchell.
pubmed:affiliation
Department of Zoology, University of Bergen, Allègaten, 41, N-5007 Bergen, Norway. bendik.terjesen@akvaforsk.nhl.no
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't