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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2001-7-2
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pubmed:abstractText |
Microperoxidase 8 (MP8) is a heme octapeptide obtained by hydrolytic digestion of horse heart cytochrome c. At pH below 9, the heme iron is axially coordinated to the imidazole side chain of His18 and to a water molecule. Replacement of this weak ligand by H2O2 allows the formation of high-valent iron-oxo species which are responsible for both peroxidase-like and cytochrome P450-like activities of MP8. This paper shows that MP8 is able to catalyze the nitration of phenol by nitrite. The reaction requires H2O2 and is inhibited by ligands having a high affinity for the iron, catalase and radical scavengers. This suggests that the nitrating species could be NO2* radicals formed by the oxidation of nitrite by high-valent iron-oxo species. This new activity of MP8 opens a new access to nitro-aromatic compounds under mild conditions and validates the use of this minienzyme to mimick heme peroxidases, especially in the reactions of NO-derived species with biomolecules under oxidative stress conditions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3783-8
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:11432746-Animals,
pubmed-meshheading:11432746-Catalysis,
pubmed-meshheading:11432746-Cytochrome c Group,
pubmed-meshheading:11432746-Free Radicals,
pubmed-meshheading:11432746-Horses,
pubmed-meshheading:11432746-Hydrogen Peroxide,
pubmed-meshheading:11432746-Hydrogen-Ion Concentration,
pubmed-meshheading:11432746-Kinetics,
pubmed-meshheading:11432746-Models, Chemical,
pubmed-meshheading:11432746-Myocardium,
pubmed-meshheading:11432746-Nitrates,
pubmed-meshheading:11432746-Nitrogen Dioxide,
pubmed-meshheading:11432746-Peroxidases,
pubmed-meshheading:11432746-Phenol,
pubmed-meshheading:11432746-Sodium Nitrite
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pubmed:year |
2001
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pubmed:articleTitle |
Microperoxidase 8 catalyzed nitration of phenol by nitrogen dioxide radicals.
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pubmed:affiliation |
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, UMR 8601 CNRS, Université Paris V, France.
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pubmed:publicationType |
Journal Article
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