Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-6-19
pubmed:abstractText
Orexin-A is synthesized in the posterolateral hypothalamus and immunoreactive fibres project to many central nervous system structures, including the paraventricular nucleus, which is rich in corticotropin releasing factor (CRF) neurones and neuropeptide Y (NPY) innervation. We investigated the central effects of orexin-A on the hypothalamic-pituitary-adrenal (HPA) axis by measuring plasma concentrations of corticosterone and adrenocorticotropic hormone (ACTH) in vivo. We explored the potential neuropeptide pathways involved by investigating the effects of orexin-A on CRF, NPY, arginine vasopressin (AVP) and noradrenaline release from hypothalamic explants in vitro. Intracerebroventricular (i.c.v.) injection of orexin-A (3 nmol) in male rats stimulated increases in plasma concentrations of corticosterone between 10 and 40 min after injection, and of plasma ACTH at 20 and 90 min after injection. Orexin-A significantly stimulated CRF and NPY release from hypothalamic explants in vitro. Orexin-A did not stimulate CRF release in the presence of the selective NPY Y1 receptor antagonist, BIBP3226. BIBP3226 alone did not alter CRF release from hypothalamic explants. Orexin-A had no effect in vitro on the release of other neuropeptides, AVP and noradrenaline, involved in the central regulation of the HPA axis. These results suggest that orexin-A is involved in activation of the HPA axis, and that these effects could be mediated via the release of NPY.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone, http://linkedlifedata.com/resource/pubmed/chemical/Arginine, http://linkedlifedata.com/resource/pubmed/chemical/Arginine Vasopressin, http://linkedlifedata.com/resource/pubmed/chemical/BIBP 3226, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Corticosterone, http://linkedlifedata.com/resource/pubmed/chemical/Corticotropin-Releasing Hormone, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptide Y, http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides, http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine, http://linkedlifedata.com/resource/pubmed/chemical/orexins
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0953-8194
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
561-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11412343-Adrenal Glands, pubmed-meshheading:11412343-Adrenocorticotropic Hormone, pubmed-meshheading:11412343-Animals, pubmed-meshheading:11412343-Arginine, pubmed-meshheading:11412343-Arginine Vasopressin, pubmed-meshheading:11412343-Carrier Proteins, pubmed-meshheading:11412343-Corticosterone, pubmed-meshheading:11412343-Corticotropin-Releasing Hormone, pubmed-meshheading:11412343-Culture Techniques, pubmed-meshheading:11412343-Hypothalamus, pubmed-meshheading:11412343-Injections, Intraventricular, pubmed-meshheading:11412343-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:11412343-Kinetics, pubmed-meshheading:11412343-Male, pubmed-meshheading:11412343-Neuropeptide Y, pubmed-meshheading:11412343-Neuropeptides, pubmed-meshheading:11412343-Norepinephrine, pubmed-meshheading:11412343-Pituitary Gland, pubmed-meshheading:11412343-Rats, pubmed-meshheading:11412343-Rats, Wistar
pubmed:year
2001
pubmed:articleTitle
The central effects of orexin-A in the hypothalamic-pituitary-adrenal axis in vivo and in vitro in male rats.
pubmed:affiliation
ICSM Endocrine Unit, ICSM, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't