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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-6-11
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pubmed:abstractText |
Rhodobacter capsulatus grew by using either L- or D-malate as carbon sources under light/anaerobic conditions. The cellular yields were the same with D- or L-malate. Both L-malate dehydrogenase and L-malic enzyme activities were detected in cell-free extracts from cells grown in both isomers. By contrast, a racemase activity converting D-malate into L-malate was induced only when D-malate was present in the culture medium. This racemase activity was Mn2+-dependent and was measured by coupling it either to the malate dehydrogenase or to the fumarase activities. The racemase activity was partially purified by anion-exchange chromatography.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0343-8651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
154-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11400062-Chromatography, Ion Exchange,
pubmed-meshheading:11400062-Culture Media,
pubmed-meshheading:11400062-Enzyme Induction,
pubmed-meshheading:11400062-Malate Dehydrogenase,
pubmed-meshheading:11400062-Malates,
pubmed-meshheading:11400062-Manganese,
pubmed-meshheading:11400062-NAD,
pubmed-meshheading:11400062-Oxidation-Reduction,
pubmed-meshheading:11400062-Racemases and Epimerases,
pubmed-meshheading:11400062-Rhodobacter capsulatus,
pubmed-meshheading:11400062-Stereoisomerism
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pubmed:year |
2001
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pubmed:articleTitle |
Assimilation of D-malate by Rhodobacter capsulatus E1F1.
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pubmed:affiliation |
Departamento de Bioquímica y Biología Molecular, Campus de Rabanales, Edificio C6, 1 Planta, 14071 Córdoba, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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