Linked Life Data

11387297

Source:http://linkedlifedata.com/resource/pubmed/id/11387297

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-6-1
pubmed:abstractText
The role of glucose fluctuates during preimplantation mouse embryo development, indicating that a specific interplay exists between glucose metabolism and uptake. In this study, attempts were made to characterize the role of the Na(+)-coupled active and the facilitated glucose transporters (GLUT) during preimplantation development by using specific glucose analogues and transport inhibitors and by examining the expression of GLUT1. One-cell outbred mouse embryos were cultured in medium M16 (5.5 mmol/l glucose), M16 without glucose (M16-G), M16-G + 2-deoxyglucose, M16-G + 3-O-methylglucose, M16 + phlorizin and M16 + phloretin and development to the blastocyst stage assessed. The absence of glucose, or the presence of 3-O-methylglucose, which is taken up but not metabolized, did not inhibit blastocyst development. 2-Deoxyglucose, which is phosphorylated but not metabolized, inhibited blastocyst development. Culture in M16 supplemented with phlorizin, an inhibitor of Na(+)-coupled active glucose transport did not inhibit blastocyst formation. Phloretin had no effect on the cleavage of two-cell embryos to the four-cell stage, but inhibited the morula/blastocyst transition. Both phloretin and phlorizin inhibited glucose uptake in two-cell embryos. Finally, GLUT1 expression was 10-fold less in blastocysts cultured in M16 compared to in-vivo blastocysts and those cultured in M16-G. The results show that both types of glucose transporters influence preimplantation embryo development and that the embryo has an innate ability to control the uptake of glucose by regulating the expression of GLUT1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1229-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11387297-3-O-Methylglucose, pubmed-meshheading:11387297-Animals, pubmed-meshheading:11387297-Biological Transport, Active, pubmed-meshheading:11387297-Blastocyst, pubmed-meshheading:11387297-Culture Media, pubmed-meshheading:11387297-Culture Techniques, pubmed-meshheading:11387297-Deoxyglucose, pubmed-meshheading:11387297-Embryonic Development, pubmed-meshheading:11387297-Embryonic and Fetal Development, pubmed-meshheading:11387297-Female, pubmed-meshheading:11387297-Glucose, pubmed-meshheading:11387297-Glucose Transporter Type 1, pubmed-meshheading:11387297-Mice, pubmed-meshheading:11387297-Monosaccharide Transport Proteins, pubmed-meshheading:11387297-Morula, pubmed-meshheading:11387297-Phloretin, pubmed-meshheading:11387297-Phlorhizin, pubmed-meshheading:11387297-Pregnancy, pubmed-meshheading:11387297-Tritium
pubmed:year
2001
pubmed:articleTitle
Facilitated glucose transporters play a crucial role throughout mouse preimplantation embryo development.
pubmed:affiliation
Clinic of Sterility, Department of Obstetrics and Gynaecology, University Hospital of Geneva, 1211 Geneva 14, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't