Source:http://linkedlifedata.com/resource/pubmed/id/11378864
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2001-5-29
|
pubmed:abstractText |
Violet laser diodes have recently become commercially available. These devices emit 5-25 mW in the range of 395-415 nm, and are available in systems that incorporate the diodes with collimating optics and regulated power supplies in housing incorporating thermoelectric coolers, which are necessary to maintain stable output. Such systems now cost several thousand dollars, but are expected to drop substantially in price. Materials and Methods A 4-mW, 397-nm violet diode system was used in a laboratory-built flow cytometer to excite fluorescence of DAPI and Hoechst dyes in permeabilized and intact cells. Forward and orthogonal light scattering were also measured.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0196-4763
|
pubmed:author | |
pubmed:copyrightInfo |
Copyright 2001 Wiley-Liss, Inc.
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
44
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
133-6
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:11378864-Cell Nucleus,
pubmed-meshheading:11378864-DNA,
pubmed-meshheading:11378864-Flow Cytometry,
pubmed-meshheading:11378864-Fluorescent Dyes,
pubmed-meshheading:11378864-Humans,
pubmed-meshheading:11378864-Jurkat Cells,
pubmed-meshheading:11378864-Lasers,
pubmed-meshheading:11378864-Light,
pubmed-meshheading:11378864-Staining and Labeling
|
pubmed:year |
2001
|
pubmed:articleTitle |
Violet laser diodes as light sources for cytometry.
|
pubmed:affiliation |
Howard M. Shapiro, M.D., P.C., West Newton Massachusetts 02465-2513, USA. hms@shapirolab.com
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|