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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-5-23
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pubmed:abstractText |
Four doubly spin-labeled variants of human carbonic anhydrase II and corresponding singly labeled variants were prepared by site-directed spin labeling. The distances between the spin labels were obtained from continuous-wave electron paramagnetic resonance spectra by analysis of the relative intensity of the half-field transition, Fourier deconvolution of line-shape broadening, and computer simulation of line-shape changes. Distances also were determined by four-pulse double electron-electron resonance. For each variant, at least two methods were applicable and reasonable agreement between methods was obtained. Distances ranged from 7 to 24 A. The doubly spin-labeled samples contained some singly labeled protein due to incomplete labeling. The sensitivity of each of the distance determination methods to the non-interacting component was compared.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-10074363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-10413479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-10479565,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-1657158,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-1909971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-1910042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-1932029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-3151019,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-7612602,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11371461-9890926
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2886-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2001
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pubmed:articleTitle |
Comparison of electron paramagnetic resonance methods to determine distances between spin labels on human carbonic anhydrase II.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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