Source:http://linkedlifedata.com/resource/pubmed/id/11358828
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2001-5-18
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| pubmed:abstractText |
Peptides presented by HLA-A*0201 molecules on the surface of the human breast carcinoma cell line KS24.22 after IFN-gamma induction were analyzed by the "Predict-Calibrate-Detect" approach, which combines epitope prediction and high-performance liquid chromatography mass spectrometry. One of the predicted epitopes, MAGE-A1(278-286) (KVLEYVIKV), was found to be presented by HLA-A*0201, with an estimated copy number of 18 molecules/cell. HLA-A*0201 transgenic mice (HHD mice) were used to generate CTL lines that stained positive with an HLA-A*0201 tetramer folded around the KVLEYVIKV peptide and killed peptide-loaded mouse target cells expressing HLA-A*0201. IFN-gamma-treated or -nontreated HLA-A*0201 expressing HeLa cells transiently transfected with a plasmid expressing the MAGE-A1 gene stimulated in vitro cytokine production by the CTL lines. Moreover, IFN-gamma-treated KS24.22 cells, but not IFN-gamma-treated HLA-A*0201(+) MAGE-A1(-) cells or IFN-gamma-treated HLA-A*0201(-) MAGE-A1(+) cells, were killed by these CTLS: Thus, the combination of HLA epitope prediction, peptide analysis, and immunological methods is a powerful approach for the identification of tumor-associated epitopes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Melanoma-Specific Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0008-5472
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
|
| pubmed:volume |
61
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4072-7
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11358828-Amino Acid Sequence,
pubmed-meshheading:11358828-Animals,
pubmed-meshheading:11358828-Antigens, Neoplasm,
pubmed-meshheading:11358828-Breast Neoplasms,
pubmed-meshheading:11358828-Chromatography, High Pressure Liquid,
pubmed-meshheading:11358828-Epitopes, T-Lymphocyte,
pubmed-meshheading:11358828-HLA-A2 Antigen,
pubmed-meshheading:11358828-HeLa Cells,
pubmed-meshheading:11358828-Humans,
pubmed-meshheading:11358828-Mass Spectrometry,
pubmed-meshheading:11358828-Melanoma-Specific Antigens,
pubmed-meshheading:11358828-Mice,
pubmed-meshheading:11358828-Neoplasm Proteins,
pubmed-meshheading:11358828-Peptide Fragments,
pubmed-meshheading:11358828-Peptide Mapping,
pubmed-meshheading:11358828-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11358828-Transfection,
pubmed-meshheading:11358828-Tumor Cells, Cultured
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| pubmed:year |
2001
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| pubmed:articleTitle |
A MAGE-A1 HLA-A A*0201 epitope identified by mass spectrometry.
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| pubmed:affiliation |
Institut for Cell Biology, Department Immunology, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. steve.pascolo@uni-tuebingen.de
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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