Source:http://linkedlifedata.com/resource/pubmed/id/11358147
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2001-5-18
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pubmed:abstractText |
Mass spectrometry was applied to identify protein spots excised from an archived two-dimensional polyacrylamide gel that had been dried and stored for eight years at room temperature. All proteins were successfully identified. Detailed characterization of protein digests by matrix-assisted laser desorption/ionization (MALDI) peptide mapping, nanoelectrospray tandem mass spectrometry and MALDI-quadrupole time-of-flight mass spectrometry revealed no evidence of protein degradation or modifications that could hamper identification of proteins in a sequence database. The experiment with a model protein demonstrated that the pattern of tryptic peptides and the yield of individual peptides were not noticeably changed in the in-gel digest of the archived protein spot compared to the digest of the spot excised from a fresh gel. Thus, the characterization of "archived proteomes" has the potential to advance proteomic research without repeating "wet" biochemistry experiments, that had been perfected in the laboratory years ago.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0173-0835
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1194-203
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11358147-Acrylic Resins,
pubmed-meshheading:11358147-Amino Acid Sequence,
pubmed-meshheading:11358147-Humans,
pubmed-meshheading:11358147-Molecular Sequence Data,
pubmed-meshheading:11358147-Peptide Mapping,
pubmed-meshheading:11358147-Protein Processing, Post-Translational,
pubmed-meshheading:11358147-Proteome,
pubmed-meshheading:11358147-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:11358147-Trypsin
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pubmed:year |
2001
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pubmed:articleTitle |
Archived polyacrylamide gels as a resource for proteome characterization by mass spectrometry.
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pubmed:affiliation |
Peptide and Protein Group, European Molecular Biology Laboratory, Heidelberg, Germany. schevchenko@EMBL-Heidelberg.de
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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