Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-5-14
pubmed:abstractText
Extracellular nucleotides cause elevation of cytosolic free Ca2+ concentration ([Ca2+](i)) in osteoclasts, although the sources of Ca2+ are uncertain. Activation of P2Y receptors causes Ca2+ release from stores, whereas P2X receptors are ligand-gated channels that mediate Ca2+ influx in some cell types. To examine the sources of Ca2+, we studied osteoclasts from rat and rabbit using fura 2 fluorescence and patch clamp. Nucleotide-induced rise of ([Ca2+](i)) persisted on removal of extracellular Ca2+ (Ca), indicating involvement of stores. Inhibition of phospholipase C (PLC) with U-73122 or inhibition of endoplasmic reticulum Ca(2+)-ATPase with cyclopiazonic acid or thapsigargin abolished the rise of ([Ca2+](i)). After store depletion in the absence of Ca, addition of Ca led to a rise of ([Ca2+](i)) consistent with store-operated Ca2+ influx. Store-operated Ca2+ influx was greater at negative potentials and was blocked by La(3+). In patch-clamp studies where PLC was blocked, ATP induced inward current indicating activation of P2X(4) nucleotide receptors, but with no rise of ([Ca2+](i)). We conclude that nucleotide-induced elevation of [Ca(2+)](i) in osteoclasts arises primarily through activation of P2Y nucleotide receptors, leading to release of Ca2+ from intracellular stores.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie..., http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases, http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents, http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Estrenes, http://linkedlifedata.com/resource/pubmed/chemical/Indoles, http://linkedlifedata.com/resource/pubmed/chemical/P2rx4 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/P2ry2 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X4, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2Y2, http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases, http://linkedlifedata.com/resource/pubmed/chemical/cyclopiazonic acid
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
280
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1531-9
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11350748-Adenosine Triphosphate, pubmed-meshheading:11350748-Animals, pubmed-meshheading:11350748-Biological Transport, pubmed-meshheading:11350748-Calcium, pubmed-meshheading:11350748-Calcium-Transporting ATPases, pubmed-meshheading:11350748-Chelating Agents, pubmed-meshheading:11350748-Cytosol, pubmed-meshheading:11350748-Egtazic Acid, pubmed-meshheading:11350748-Enzyme Inhibitors, pubmed-meshheading:11350748-Estrenes, pubmed-meshheading:11350748-Femur, pubmed-meshheading:11350748-Indoles, pubmed-meshheading:11350748-Mammals, pubmed-meshheading:11350748-Membrane Potentials, pubmed-meshheading:11350748-Osteoclasts, pubmed-meshheading:11350748-Patch-Clamp Techniques, pubmed-meshheading:11350748-Phosphodiesterase Inhibitors, pubmed-meshheading:11350748-Pyrrolidinones, pubmed-meshheading:11350748-Rabbits, pubmed-meshheading:11350748-Rats, pubmed-meshheading:11350748-Receptors, Purinergic P2, pubmed-meshheading:11350748-Receptors, Purinergic P2X4, pubmed-meshheading:11350748-Receptors, Purinergic P2Y2, pubmed-meshheading:11350748-Thapsigargin, pubmed-meshheading:11350748-Tibia, pubmed-meshheading:11350748-Type C Phospholipases
pubmed:year
2001
pubmed:articleTitle
Activation of P2Y but not P2X(4) nucleotide receptors causes elevation of [Ca2+]i in mammalian osteoclasts.
pubmed:affiliation
Department of Physiology and Division of Oral Biology, Faculty of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5C1.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't