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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2001-5-8
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pubmed:abstractText |
Red blood cells (RBCs) are known to perform one prominent function: to carry and deliver oxygen to the tissues. Earlier studies, however, suggested a role for RBCs in potentiating T-cell proliferation in vitro. Here it is shown that the presence of RBCs in cultures of stimulated human peripheral blood lymphocytes strengthens T-cell proliferation and survival. Analysis of phosphatidylserine externalization and DNA fragmentation showed that RBCs inhibit T-cell apoptosis. This inhibition correlated with a reduction in CD71 but not CD95 expression. RBCs enhanced T-cell proliferation and survival upon activation with phytohemagglutinin and with OKT3 antibodies. Studies aimed at characterizing the cellular and molecular basis of the protection afforded to T cells by RBCs showed that (1) optimal protection required intact RBCs and red cell/T-cell contact but not monocytes; (2) RBCs markedly reduced the level of intracellular reactive oxygen species; and (3) RBCs inhibited the formation of protein-bound acrolein, a peroxidation adduct in biologic systems. Overall, these data indicate that human RBCs protect T cells from activation-induced cell death, at least in part by reducing the pro-oxidant state, and suggest a role for RBCs as conceivable modulators of T-cell homeostasis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acrolein,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD71 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylserines,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3152-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11342443-Acrolein,
pubmed-meshheading:11342443-Antibodies, Monoclonal,
pubmed-meshheading:11342443-Antigens, CD,
pubmed-meshheading:11342443-Antigens, CD95,
pubmed-meshheading:11342443-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:11342443-Apoptosis,
pubmed-meshheading:11342443-Cell Division,
pubmed-meshheading:11342443-Cell Survival,
pubmed-meshheading:11342443-Coculture Techniques,
pubmed-meshheading:11342443-DNA Fragmentation,
pubmed-meshheading:11342443-Erythrocytes,
pubmed-meshheading:11342443-Humans,
pubmed-meshheading:11342443-Lymphocyte Activation,
pubmed-meshheading:11342443-Monocytes,
pubmed-meshheading:11342443-Oxidative Stress,
pubmed-meshheading:11342443-Phosphatidylserines,
pubmed-meshheading:11342443-Phytohemagglutinins,
pubmed-meshheading:11342443-Reactive Oxygen Species,
pubmed-meshheading:11342443-Receptors, Transferrin,
pubmed-meshheading:11342443-T-Lymphocytes
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pubmed:year |
2001
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pubmed:articleTitle |
Red blood cells inhibit activation-induced cell death and oxidative stress in human peripheral blood T lymphocytes.
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pubmed:affiliation |
Laboratory of Molecular Immunology, Institute for Molecular and Cell Biology, University of Porto, Portugal.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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