Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-5-8
pubmed:abstractText
The genotoxicity of river water and sediment including interstitial water was evaluated by microscreen phage-induction and Salmonella/microsome assays. Different processes used to fractionate the sediment sample were compared using solvents with different polarities. The results obtained for mutagenic activity using the Salmonella/microsome test were negative in the water and interstitial water samples analysed using the direct concentration method. The responses in the microscreen phage-induction assay showed the presence of genotoxic or indicative genotoxic activity for at least one water sample of each site analysed using the same concentration method. Similar results were obtained for interstitial water samples, i.e. absence of mutagenic activity in the Salmonella/microsome test and presence of genotoxic activity in the microscreen phage-induction assay. Metal contamination, as evidenced by the concentrations in stream sediments, may also help explain some of these genotoxic results. Stream sediment organic extracts showed frameshift mutagenic activity in the ether extract detected by Salmonella/microsome assay. The concentrates evaluated by microscreen phage-induction assay identified the action of organic compounds in the non-polar, medium polar and polar fractions. Thus, the microscreen phage-induction assay has proven to be a more appropriate methodology than the Salmonella/microsome test to analyse multiple pollutants in this ecosystem where both organic compounds and heavy metals are present.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
490
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-58
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11342240-Animals, pubmed-meshheading:11342240-Bacteriophage lambda, pubmed-meshheading:11342240-Biotransformation, pubmed-meshheading:11342240-Brazil, pubmed-meshheading:11342240-DNA, Bacterial, pubmed-meshheading:11342240-Escherichia coli, pubmed-meshheading:11342240-Fresh Water, pubmed-meshheading:11342240-Genes, Bacterial, pubmed-meshheading:11342240-Geologic Sediments, pubmed-meshheading:11342240-Industrial Waste, pubmed-meshheading:11342240-Lysogeny, pubmed-meshheading:11342240-Male, pubmed-meshheading:11342240-Metals, Heavy, pubmed-meshheading:11342240-Microsomes, Liver, pubmed-meshheading:11342240-Mutagenicity Tests, pubmed-meshheading:11342240-Rats, pubmed-meshheading:11342240-Rats, Sprague-Dawley, pubmed-meshheading:11342240-SOS Response (Genetics), pubmed-meshheading:11342240-Salmonella typhimurium, pubmed-meshheading:11342240-Solvents, pubmed-meshheading:11342240-Virus Activation, pubmed-meshheading:11342240-Water Pollutants, Chemical, pubmed-meshheading:11342240-Water Pollution, Chemical
pubmed:year
2001
pubmed:articleTitle
Genotoxicity assessment in aquatic environments under the influence of heavy metals and organic contaminants.
pubmed:affiliation
Fundação Estadual de Proteção Ambiental Henrique Luis FEPAM, Av. Salvador França, 1707, 90690-000 Porto Alegre, RS, Brazil. vvargas@pro.vias-rs.com.br
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't