Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2001-5-4
pubmed:abstractText
Human tear lipocalin [lipocalin 1 (lcn-1); von Ebner's gland protein] is a member of the lipocalin superfamily that is known to bind an unusual variety of lipophilic ligands. Because of its properties and its tissue-specific expression it has been suggested that lcn-1 might act as a physiological protection factor of epithelia. Overexpression of lcn-1 under certain disease conditions supported such a function. However, experimental investigations into its exact biological role and its mode of expression were impeded because lcn-1 was previously found to be produced only in serous glands. To overcome this problem we therefore sought a cell line that produced lcn-1 endogenously. Using reverse-transcriptase-mediated PCR analysis we found expression of lcn-1 in the human teratocarcinoma-derived NT2 precursor cells. Under normal conditions the production of lcn-1 is low. However, treatment of the cells with H(2)O(2) or FeSO(4), which typically induce lipid peroxidation, significantly enhanced the expression of lcn-1. Binding studies revealed that arachidonic acid and several lipid peroxidation products including 7beta-hydroxycholesterol, 8-isoprostane and 13-hydroxy-9,11-octadecadienoic acid specifically bind to lcn-1. To investigate the physiological consequence of this observation we purified holo-(lcn-1) from culture medium and extracted the bound ligands. The presence of F(2)-isoprostanes in the extracts obtained from the fractions containing lcn-1 indicates that these typical lipid peroxidation products are indeed ligands of the protein in vivo. These results support the idea that lcn-1 acts as a physiological scavenger of potentially harmful lipophilic molecules; lcn-1 might therefore be a novel member of the cellular defence against the deleterious effects of oxidative stress.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10415451, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10455102, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10515687, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10571084, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10586930, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10607840, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10612294, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10769187, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-10825538, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-11058765, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-1400345, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-1510819, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-1696009, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-1698296, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-1709540, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-1931961, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-2123555, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-2233347, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-2717374, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-4171938, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-6194713, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7526418, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7648862, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7679926, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7700313, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7789516, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7813422, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-7878087, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8112601, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8125951, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8136014, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8168376, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8478929, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8500570, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8898072, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8912185, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8978487, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-8999869, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-9458722, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-9640200, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-9717713, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-9759656, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-9790888, http://linkedlifedata.com/resource/pubmed/commentcorrection/11336644-9830672
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/11-(dansylamino)undecanoic acid, http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Dansyl Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Dinoprost, http://linkedlifedata.com/resource/pubmed/chemical/F2-Isoprostanes, http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes, http://linkedlifedata.com/resource/pubmed/chemical/LCN1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Lipid Peroxides, http://linkedlifedata.com/resource/pubmed/chemical/Lipocalin 1, http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
356
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-35
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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