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rdf:type |
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lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0025252,
umls-concept:C0031715,
umls-concept:C0033684,
umls-concept:C0085732,
umls-concept:C0116397,
umls-concept:C0205145,
umls-concept:C0205164,
umls-concept:C0205263,
umls-concept:C0205275,
umls-concept:C0851285,
umls-concept:C1519063,
umls-concept:C2242140
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pubmed:issue |
11
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pubmed:dateCreated |
2001-5-3
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pubmed:abstractText |
Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) is a phosphoprotein suggested to play important roles in EBV-induced immortalization of B cells. One of the potential functions of EBNA-LP is a cooperative induction with EBNA-2 of viral and cellular gene expression, including that of the genes for viral latent membrane protein 1 (LMP-1) and cellular cyclin D2. We report here that the phosphorylation of EBNA-LP by cellular kinase(s) is critical to its ability to cooperate with EBNA-2 in up-regulating the expression of LMP-1 in a B-lymphoma cell line. Our conclusion is based on the following observations. (i) Mass-spectrometric analysis of purified EBNA-LP and mutational analyses of EBNA-LP revealed that the serine residue at position 35 in the W2 repeat domain is the major phosphorylation site of EBNA-LP in vivo. (ii) Substitutions of this site in each W2 repeat domain with alanine markedly reduced the ability of the protein to induce LMP-1 expression in combination with EBNA-2 in Akata cells. (iii) Replacement at the major phosphorylation sites with glutamic acids restored the wild-type phenotype. It is well established that this substitution mimics constitutive phosphorylation. These results indicated that the coactivator function of EBNA-LP is regulated by phosphorylation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-10448085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-10590127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-10708424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-10724175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-11024123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-11024139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-11028910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-11162796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-1451779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-1658376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-1659990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-1956339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-2161150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-2462063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-2547164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-2824821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-2845663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-2956925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-6087149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-6091123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-6202700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-7494344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-7561751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-7690960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-8045261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-8390666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-8642686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-8659132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-8892911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-8995621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9058808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9211847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9261383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9261384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9311776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9311810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9499021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9499396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11333893-9823900
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/EBNA-2 protein, Human herpesvirus 4,
http://linkedlifedata.com/resource/pubmed/chemical/EBNA-LP protein, Human herpesvirus 4,
http://linkedlifedata.com/resource/pubmed/chemical/EBV-associated membrane antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
pubmed-author:HiraiKK,
pubmed-author:HiranoHH,
pubmed-author:KanamoriMM,
pubmed-author:KatoKK,
pubmed-author:KawaguchiYY,
pubmed-author:KawasakiHH,
pubmed-author:KitabayashiII,
pubmed-author:MatsudaGG,
pubmed-author:OhkiMM,
pubmed-author:TanakaMM,
pubmed-author:YokoyamaAA
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5119-28
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11333893-Humans,
pubmed-meshheading:11333893-Animals,
pubmed-meshheading:11333893-Alanine,
pubmed-meshheading:11333893-Glutamic Acid,
pubmed-meshheading:11333893-Phosphorylation,
pubmed-meshheading:11333893-Phosphotransferases,
pubmed-meshheading:11333893-Viral Proteins,
pubmed-meshheading:11333893-Burkitt Lymphoma,
pubmed-meshheading:11333893-Binding Sites,
pubmed-meshheading:11333893-Cell Line,
pubmed-meshheading:11333893-Mass Spectrometry,
pubmed-meshheading:11333893-Herpesvirus 4, Human
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