Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-5-1
pubmed:abstractText
An immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) was developed for the detection of primary infection of vesicular stomatitis virus (VSV) in equine and swine sera. The test was based on the use of biotinylated sheep antibodies against equine or swine IgM molecules bound to a streptavidin-coated ELISA plate. The captured IgM antibodies were detected by application of antigens prepared from the New Jersey and the Indiana VSV serotypes (VSV-NJ and VSV-IN, respectively) and mouse polyclonal antibodies against VSV-NJ and VSV-IN. The MC-ELISA was compared to a competitive ELISA (C-ELISA) and the standard microtiter serum neutralization (MTSN) assay by testing serum samples from horses and pigs experimentally infected with VSV-NJ or VSV-IN. The MC-ELISA detected specific homologous IgM antibodies from equine and swine sera as early as 5 and 4 days postinfection (DPI), respectively, and as late as 35 DPI. The MTSN test also detected antibodies as early as 5 DPI and as late as 160 DPI. In a similar fashion, the C-ELISA detected antibodies from 6 to 7 DPI and as late as 160 DPI. These results demonstrated that the MC-ELISA is a useful test for serodiagnosis of primary VSV infection in horses and pigs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-12987386, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-13345692, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-3001132, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-4062563, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-500329, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-6337233, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-7710096, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-8385501, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-8394377, http://linkedlifedata.com/resource/pubmed/commentcorrection/11329443-9249179
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
475-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus.
pubmed:affiliation
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada. ezhou@iastate.edu
pubmed:publicationType
Journal Article