Source:http://linkedlifedata.com/resource/pubmed/id/11327744
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-4-30
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| pubmed:abstractText |
This study was performed to compare the effects of recombinant human growth hormone (rhGH), glutamine (Gln) and simultaneous treatment with rhGH and Gln in rats subjected to 75% intestinal resection and maintained with parenteral nutrition (PN) for 6 days. Morphological changes including mucosal thickness, villus height, crypt depths and villus surface area of the residue jejunum were measured under a light microscope; expression of PCNA as an index of cell proliferation and apoptotic cells were observed using immunohistochemical staining; Ileal IGF-1 mRNA was determined by Northern blot analysis. The morphological parameters of the jejunal mucosa in rats treated with PN alone were only about 52-62% of those in reference group (P<0.01), this atrophy of the jejunal mucosa was accompanied by a 2.5-fold decrease in absolute counts of PCNA and a 10-fold increase in apoptotic index (P<0.01), IGF-1 mRNA transcript in residue ileum was decreased significantly (P<0.01). However, with rhGH or Gln, the mucosal architecture was improved significantly and was further improved when rhGH and Gln were given together, the morphological values in rats treated with Gln+rhGH was 79% higher than those with PN alone, and was associated with a 2-fold increase in PCNA counts and a 4-fold decrease in apoptotic index (P<0.01), IGF-1 mRNA expression was 78% higher than those with PN alone (P<0.01). We conclude that rhGH and Gln have synergistic effects on adaptation of the intestinal remnant in parenterally fed, short-bowel rats. The underlying mechanisms are associated with increased proliferation and decreased apoptosis in the intestinal epithelial cells. Local intestinal production of IGF-1 plays an important role in adaptation of the small intestine. Our findings support the concept that specific gut-trophic nutrients and growth factors may be combined to enhance the intestinal adaptation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0261-5614
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Harcourt Publishers Ltd.
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| pubmed:issnType |
Print
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| pubmed:volume |
20
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
159-66
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11327744-Adaptation, Biological,
pubmed-meshheading:11327744-Animals,
pubmed-meshheading:11327744-Apoptosis,
pubmed-meshheading:11327744-Atrophy,
pubmed-meshheading:11327744-Dietary Supplements,
pubmed-meshheading:11327744-Drug Synergism,
pubmed-meshheading:11327744-Glutamine,
pubmed-meshheading:11327744-Growth Hormone,
pubmed-meshheading:11327744-Immunohistochemistry,
pubmed-meshheading:11327744-Insulin-Like Growth Factor I,
pubmed-meshheading:11327744-Intestinal Mucosa,
pubmed-meshheading:11327744-Intestines,
pubmed-meshheading:11327744-Male,
pubmed-meshheading:11327744-Parenteral Nutrition,
pubmed-meshheading:11327744-RNA, Messenger,
pubmed-meshheading:11327744-Random Allocation,
pubmed-meshheading:11327744-Rats,
pubmed-meshheading:11327744-Rats, Sprague-Dawley,
pubmed-meshheading:11327744-Short Bowel Syndrome
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| pubmed:year |
2001
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| pubmed:articleTitle |
Effects of growth hormone (rhGH) and glutamine supplemented parenteral nutrition on intestinal adaptation in short bowel rats.
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| pubmed:affiliation |
Department of Surgery, Zhongshan Hospital, Shanghai, 200032, PR China.
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| pubmed:publicationType |
Journal Article
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