Linked Life Data

11322764

Source:http://linkedlifedata.com/resource/pubmed/id/11322764

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-4-27
pubmed:abstractText
We conducted the following experiments to determine whether curcumin, an antioxidant compound extracted from the spice tumeric, inhibits cell death induced by Shiga toxin (Stx) 1 and 2 in HK-2 cells, a human proximal tubule cell line. Cells were incubated for 24-48 h with Stx1 or Stx2, 0-100 ng/ml. Test media contained either no further additives or 10-50 microM curcumin. Exposure to Stx1 and Stx2, 100 ng/ml, reduced cell viability to approximately 25% of control values after 24 h and 20 microM curcumin restored viability to nearly 75% of control. Cell staining confirmed that Stx1 and Stx2-induced damage in HK-2 cells involved a combination of apoptosis and necrosis. Thus, Stx1 caused apoptosis and necrosis in 12.2 +/- 2.2 and 12.7 +/- 0.9% of HK-2 cells, respectively. Similarly, Stx2 caused apoptosis and necrosis in 13.4 +/- 2.1 and 9.0 +/- 0.5% of HK-2 cells, respectively. Addition of 20 microM curcumin decreased the extent of apoptosis and necrosis to 2.9 +/- 2.0 and 3.8 +/- 0.2%, respectively in the presence of Stx1 and to 3.0 +/- 2.1 and 3.9 +/- 0.3%, respectively, for Stx2 (P < 0.01). Stx-induced apoptosis and its inhibition by curcumin were confirmed by DNA gel electrophoresis and by an assay for fragmentation. The protective effect of curcumin against Stx1 and Stx2-induced injury to HK-2 was not related to its antioxidant properties. Instead, curcumin enhanced expression of heat shock protein 70 (HSP70) in HK-2 cells under control conditions and after exposure to Stx1 or Stx2. No injury was detectable after incubation of LLC-PK(1) or OK cells, non-human proximal tubule cell lines, with Stx1 or Stx2. Thus, curcumin inhibits Stx-induced apoptosis and necrosis in HK-2 cells in vitro. The cytoprotective effect of curcumin against Stx-induced injury in cultured human proximal tubule epithelial cells may be a consequence of increased expression of HSP70.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-41
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11322764-Animals, pubmed-meshheading:11322764-Antioxidants, pubmed-meshheading:11322764-Apoptosis, pubmed-meshheading:11322764-Blotting, Western, pubmed-meshheading:11322764-Cell Line, pubmed-meshheading:11322764-Cell Survival, pubmed-meshheading:11322764-Curcumin, pubmed-meshheading:11322764-Cytoprotection, pubmed-meshheading:11322764-DNA Fragmentation, pubmed-meshheading:11322764-Enzyme Inhibitors, pubmed-meshheading:11322764-Epithelial Cells, pubmed-meshheading:11322764-HSP70 Heat-Shock Proteins, pubmed-meshheading:11322764-Humans, pubmed-meshheading:11322764-Kidney Tubules, Proximal, pubmed-meshheading:11322764-LLC-PK1 Cells, pubmed-meshheading:11322764-Necrosis, pubmed-meshheading:11322764-Shiga Toxin 1, pubmed-meshheading:11322764-Shiga Toxin 2, pubmed-meshheading:11322764-Shiga Toxins, pubmed-meshheading:11322764-Swine
pubmed:year
2001
pubmed:articleTitle
Cytoprotective effect of curcumin in human proximal tubule epithelial cells exposed to shiga toxin.
pubmed:affiliation
Department of Pediatrics, Schneider Children's Hospital of the North Shore-Long Island Jewish Health System, Albert Einstein College of Medicine, New Hyde Park, New York, 11040-1432, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.