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rdf:type |
|
|
lifeskim:mentions |
|
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pubmed:issue |
2
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pubmed:dateCreated |
2001-4-24
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|
pubmed:abstractText |
The envelope glycoproteins, surface unit (SU) and transmembrane (TM) of the murine leukemia virus (MLV) are not covalently linked and tend to dissociate upon high-speed centrifugation, leading to loss of vector infectivity. This study describes a gentle and simple method to concentrate MLV vectors or HIV vectors pseudotyped with MLV envelopes. Having a fast and inexpensive method to concentrate large volumes of vector supernatant will facilitate in vivo experiments and clinical trials that require high titer vector stocks.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:issn |
1099-498X
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
|
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pubmed:authorsComplete |
Y
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pubmed:pagination |
188-94
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11318118-Animals,
pubmed-meshheading:11318118-Calcium Phosphates,
pubmed-meshheading:11318118-Cell Line,
pubmed-meshheading:11318118-Genetic Vectors,
pubmed-meshheading:11318118-Humans,
pubmed-meshheading:11318118-Hydrogen-Ion Concentration,
pubmed-meshheading:11318118-Microscopy, Electron,
pubmed-meshheading:11318118-Precipitin Tests,
pubmed-meshheading:11318118-Retroviridae,
pubmed-meshheading:11318118-Transduction, Genetic
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pubmed:articleTitle |
Concentration of viral vectors by co-precipitation with calcium phosphate.
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pubmed:affiliation |
Molecular Medicine Program, Mayo Foundation, Rochester, MN 55905, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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