Source:http://linkedlifedata.com/resource/pubmed/id/11313393
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2001-4-23
|
| pubmed:abstractText |
Ag recognition triggered at the interface between a T cell and an APC is conditioned by cell-cell adhesion and cytoskeletal remodeling. The role played in these phenomena by Lck and Itk, two protein tyrosine kinases essential for T cell signaling, was examined. Early T cell responses (membrane ruffling, Ca(2+) response, APC-T cell adhesion) were monitored in T cells overexpressing kinase-defective (KD) Lck and Itk mutants by combining fluorescence imaging and electron microscopy. Neither Lck nor Itk appears to be involved in the Ag-independent formation of a small and labile contact interface between T cells and APCS: By contrast, the Ag-induced Ca(2+) response in a cell population is similarly blunted in both KD transfectants. However, the underlying mechanisms are strikingly different for the two kinases. The major effect of Lck-KD is to reduce the probability of giving rise to quasi-normal Ca(2+) responses, whereas overexpression of Itk-KD results in a tuning down of all single-cell Ca(2+) responses. In addition, Lck, but not Itk, is required for the formation of a stable T/APC conjugate and for T cell polarization after Ag stimulation. Overall, our results lead to a clear distinction between Lck and ITK: Lck plays an ignition role, controlling all the downstream events tested here, whereas Itk amplifies the Ca(2+) response, but is dispensable for APC-induced adhesive and morphological responses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
166
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5540-9
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:11313393-Animals,
pubmed-meshheading:11313393-Antigen Presentation,
pubmed-meshheading:11313393-Antigen-Presenting Cells,
pubmed-meshheading:11313393-Calcium,
pubmed-meshheading:11313393-Calcium Signaling,
pubmed-meshheading:11313393-Cell Adhesion,
pubmed-meshheading:11313393-Cell Size,
pubmed-meshheading:11313393-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11313393-Humans,
pubmed-meshheading:11313393-Hybridomas,
pubmed-meshheading:11313393-L Cells (Cell Line),
pubmed-meshheading:11313393-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:11313393-Mice,
pubmed-meshheading:11313393-Microscopy, Electron, Scanning,
pubmed-meshheading:11313393-Microscopy, Video,
pubmed-meshheading:11313393-Protein-Tyrosine Kinases,
pubmed-meshheading:11313393-T-Lymphocytes,
pubmed-meshheading:11313393-Transfection
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Differential roles of Lck and Itk in T cell response to antigen recognition revealed by calcium imaging and electron microscopy.
|
| pubmed:affiliation |
Laboratoire d'Immuno-Pharmacologie, Centre National de la Recherche Scientifique, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|