Linked Life Data

11309699

Source:http://linkedlifedata.com/resource/pubmed/id/11309699

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-4-19
pubmed:abstractText
The capacity for multiparametric measurement of large cell populations rapidly and accurately offered by cytometry has made this methodology indispensable in studies of cell proliferation and cell death. The reviewed cell cycle applications include (1) the univariate analysis of cellular DNA content for identification of G(0/1) versus S versus G(2)/M cells; (2) discrimination between noncycling (G(0); quiescent) and proliferating cells, based on the presence of proliferation-associated proteins; (3) identification of mitotic cells by histone H3 phosphorylation; (4) bivariate analysis of expression of cyclins D, E, A, or B1 versus DNA content; and (5) detection of DNA replicating cells and analysis of cell kinetics from the bivariate distributions of 5-bromo-2'-deoxyuridine (BrdU) incorporation versus DNA content. For the identification of apoptotic cells and discrimination between apoptosis and necrosis, flow cytometry techniques are applied to evaluate for changes in cell morphology, the presence of phosphatidylserine on cell surface, collapse of mitochondrial transmembrane potential, DNA fragmentation, and evidence of caspase activation. Semin Hematol 38:179-193.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0037-1963
pubmed:author
pubmed:copyrightInfo
Copyright 2001 by W.B. Saunders Company.
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-93
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Flow cytometry in analysis of cell cycle and apoptosis.
pubmed:affiliation
Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review, Research Support, Non-U.S. Gov't