Linked Life Data

11306719

Source:http://linkedlifedata.com/resource/pubmed/id/11306719

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-4-18
pubmed:abstractText
Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, although angiogenic factor and integrin-extracellular matrix interaction modulate this process. We report here that a snake venom-derived disintegrin, rhodostomin, inhibited distinct steps in angiogenesis elicited by basic fibroblast growth factor (bFGF), and also suppressed in vivo murine melanoma tumor growth. Rhodostomin dose-dependently inhibited bFGF-induced human umbilical vein endothelial cell (HUVEC) proliferation as examined by cell number count, metabolic activity, and BrdU incorporation assays with submicromolar IC(50) values. However, it apparently did not affect the viability of murine B16F10 melanoma cells, even up to 50 microM. Rhodostomin also inhibited HUVEC migration and invasion evoked by bFGF, and tube formation of bFGF-treated HUVECs in Matrigel. Moreover, rhodostomin selectively inhibited bFGF-, but not vascular endothelial growth factor-associated angiogenesis in the chick chorioallantoic membrane model. Furthermore, rhodostomin blocked both bFGF- and B16F10-induced neovascularization in murine Matrigel plug model and suppressed the growth of subcutaneously inoculated B16F10 solid tumor, leading to a prolonged survival of the rhodostomin-treated C57BL/6 mice. The antiangiogenic effect of rhodostomin on bFGF-treated HUVECs is related to the integrin alpha(v)beta(3) blockade, as evidenced by its selective inhibition on the binding of 7E3, a monoclonal antibody (mAb) raised against alpha(v)beta(3,) but not that of P1F6, an alpha(v)beta(5) mAb toward both naive and bFGF-primed HUVECs. Moreover, 7E3 specifically blocked fluorescein isothiocyanate-conjugated rhodostomin binding to HUVEC, whereas P1F6 and anti-integrin alpha(2), alpha(3), alpha(4), or alpha(5) mAbs did not.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1333-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11306719-Angiogenesis Inhibitors, pubmed-meshheading:11306719-Animals, pubmed-meshheading:11306719-Cell Division, pubmed-meshheading:11306719-Cell Movement, pubmed-meshheading:11306719-Cell Survival, pubmed-meshheading:11306719-Cells, Cultured, pubmed-meshheading:11306719-Chick Embryo, pubmed-meshheading:11306719-Disease Models, Animal, pubmed-meshheading:11306719-Drug Interactions, pubmed-meshheading:11306719-Endothelium, Vascular, pubmed-meshheading:11306719-Fibroblast Growth Factor 2, pubmed-meshheading:11306719-Growth Substances, pubmed-meshheading:11306719-Humans, pubmed-meshheading:11306719-Mice, pubmed-meshheading:11306719-Mice, Inbred C57BL, pubmed-meshheading:11306719-Neoplasm Transplantation, pubmed-meshheading:11306719-Neoplasms, Experimental, pubmed-meshheading:11306719-Neovascularization, Pathologic, pubmed-meshheading:11306719-Neovascularization, Physiologic, pubmed-meshheading:11306719-Peptides, pubmed-meshheading:11306719-Platelet Aggregation Inhibitors, pubmed-meshheading:11306719-Receptors, Vitronectin, pubmed-meshheading:11306719-Snake Venoms
pubmed:year
2001
pubmed:articleTitle
Rhodostomin, a snake venom disintegrin, inhibits angiogenesis elicited by basic fibroblast growth factor and suppresses tumor growth by a selective alpha(v)beta(3) blockade of endothelial cells.
pubmed:affiliation
Department of Pharmacology, College of Medicine, National Taiwan University, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't