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rdf:type |
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lifeskim:mentions |
umls-concept:C0025936,
umls-concept:C0031727,
umls-concept:C0033681,
umls-concept:C0162508,
umls-concept:C0214604,
umls-concept:C0332120,
umls-concept:C0390423,
umls-concept:C0597357,
umls-concept:C1149880,
umls-concept:C1280500,
umls-concept:C1332036,
umls-concept:C1335273,
umls-concept:C1705845
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pubmed:issue |
5
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pubmed:dateCreated |
2001-4-16
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pubmed:abstractText |
Angiotensin II (Ang II) has two major receptor isoforms, AT1 and AT2. AT1 transphosphorylates Ca(2+)-sensitive tyrosine kinase Pyk2 to activate c-Jun NH2-terminal kinase (JNK). Although AT2 inactivates extracellular signal-regulated kinase (ERK) via tyrosine phosphatases (PTP), the action of AT2 on Pyk2 and JNK remains undefined. Using AT2-overexpressing vascular smooth muscle cells (AT2-VSMC) from AT2-transgenic mice, we studied these undefined actions of AT2. AT1-mediated JNK activity was increased 2.2-fold by AT2 inhibition, which was abolished by orthovanadate. AT2 did not affect AT1-mediated Pyk2 phosphorylation, but attenuated c-Jun mRNA accumulation by 32%. The activity of src-homology 2 domain-containing PTP (SHP-1) was significantly upregulated 1 min after AT2 stimulation. Stable overexpression of SHP-1 dominant negative mutant in AT2-VSMC completely abolished AT2-mediated inhibition of JNK activation and c-Jun expression. These findings suggest that AT2 inhibits JNK activity by affecting the downstream signal of Pyk2 in a SHP-1-dependent manner, leading to a decrease in c-Jun expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptk2b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ptpn6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:FujiyamaSS,
pubmed-author:HasegawaTT,
pubmed-author:HoriuchiMM,
pubmed-author:IbeKK,
pubmed-author:IwasakaTT,
pubmed-author:KosakaYY,
pubmed-author:MOEDH KHK,
pubmed-author:MasakiHH,
pubmed-author:MatsubaraHH,
pubmed-author:MoriYY,
pubmed-author:NahmiasCC,
pubmed-author:OkigakiMM,
pubmed-author:ShibasakiYY,
pubmed-author:TateishiEE,
pubmed-author:TsutsumiYY,
pubmed-author:UchiyamaYY
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1085-91
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11302725-Angiotensin Receptor Antagonists,
pubmed-meshheading:11302725-Animals,
pubmed-meshheading:11302725-Calcium,
pubmed-meshheading:11302725-Cells, Cultured,
pubmed-meshheading:11302725-Enzyme Inhibitors,
pubmed-meshheading:11302725-Focal Adhesion Kinase 2,
pubmed-meshheading:11302725-Genes, Dominant,
pubmed-meshheading:11302725-Intracellular Fluid,
pubmed-meshheading:11302725-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:11302725-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:11302725-Mice,
pubmed-meshheading:11302725-Mice, Transgenic,
pubmed-meshheading:11302725-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11302725-Muscle, Smooth, Vascular,
pubmed-meshheading:11302725-Phosphorylation,
pubmed-meshheading:11302725-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:11302725-Protein Tyrosine Phosphatase, Non-Receptor Type 6,
pubmed-meshheading:11302725-Protein Tyrosine Phosphatases,
pubmed-meshheading:11302725-Protein-Tyrosine Kinases,
pubmed-meshheading:11302725-RNA, Messenger,
pubmed-meshheading:11302725-Receptor, Angiotensin, Type 1,
pubmed-meshheading:11302725-Receptor, Angiotensin, Type 2,
pubmed-meshheading:11302725-Receptors, Angiotensin,
pubmed-meshheading:11302725-Signal Transduction,
pubmed-meshheading:11302725-Vanadates
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pubmed:year |
2001
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pubmed:articleTitle |
Effect of angiotensin II type 2 receptor on tyrosine kinase Pyk2 and c-Jun NH2-terminal kinase via SHP-1 tyrosine phosphatase activity: evidence from vascular-targeted transgenic mice of AT2 receptor.
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pubmed:affiliation |
Department of Medicine II, Kansai Medical University, Moriguchi, Osaka, 570, Japan. matsubah@takaii.kmu.ac.jp
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pubmed:publicationType |
Journal Article
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