Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-4-20
pubmed:abstractText
Several lines of evidence implicate excitotoxic mechanisms in the pathogenesis of amyotrophic lateral sclerosis (ALS). Transgenic mice with a superoxide dismutase mutation (G93A) have been utilized as an animal model of familial ALS (FALS). We examined the cortical concentrations of glutamate using in vivo microdialysis and in vivo nuclear magnetic resonance (NMR) spectroscopy, and the effect of long-term creatine supplementation. NMDA-stimulated and Ltrans-pyrrolidine-2,4-dicarboxylate (LTPD)-induced increases in glutamate were significantly higher in G93A mice compared with littermate wild-type mice at 115 days of age. At this age, the tissue concentrations of glutamate were also significantly increased as measured with NMR spectroscopy. Creatine significantly increased longevity and motor performance of the G93A mice, and significantly attenuated the increases in glutamate measured with spectroscopy at 75 days of age, but had no effect at 115 days of age. These results are consistent with impaired glutamate transport in G93A transgenic mice. The beneficial effect of creatine may be partially mediated by improved function of the glutamate transporter, which has a high demand for energy and is susceptible to oxidative stress.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters, http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG, http://linkedlifedata.com/resource/pubmed/chemical/Creatine, http://linkedlifedata.com/resource/pubmed/chemical/Dicarboxylic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Agonists, http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Glutamine, http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate, http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Uptake Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines, http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase, http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine-2,4-dicarboxylic acid
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
383-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11299300-ATP-Binding Cassette Transporters, pubmed-meshheading:11299300-Amino Acid Transport System X-AG, pubmed-meshheading:11299300-Animals, pubmed-meshheading:11299300-Biological Transport, pubmed-meshheading:11299300-Body Weight, pubmed-meshheading:11299300-Brain Chemistry, pubmed-meshheading:11299300-Creatine, pubmed-meshheading:11299300-Dicarboxylic Acids, pubmed-meshheading:11299300-Disease Models, Animal, pubmed-meshheading:11299300-Excitatory Amino Acid Agonists, pubmed-meshheading:11299300-Glutamic Acid, pubmed-meshheading:11299300-Glutamine, pubmed-meshheading:11299300-Magnetic Resonance Spectroscopy, pubmed-meshheading:11299300-Male, pubmed-meshheading:11299300-Mice, pubmed-meshheading:11299300-Mice, Transgenic, pubmed-meshheading:11299300-Microdialysis, pubmed-meshheading:11299300-Motor Activity, pubmed-meshheading:11299300-Motor Neuron Disease, pubmed-meshheading:11299300-N-Methylaspartate, pubmed-meshheading:11299300-Neurotransmitter Uptake Inhibitors, pubmed-meshheading:11299300-Oxidative Stress, pubmed-meshheading:11299300-Psychomotor Performance, pubmed-meshheading:11299300-Pyrrolidines, pubmed-meshheading:11299300-Superoxide Dismutase
pubmed:year
2001
pubmed:articleTitle
Increases in cortical glutamate concentrations in transgenic amyotrophic lateral sclerosis mice are attenuated by creatine supplementation.
pubmed:affiliation
Neurochemistry Laboratory, Neurology Service, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't