Source:http://linkedlifedata.com/resource/pubmed/id/11298793
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2001-4-12
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| pubmed:abstractText |
To investigate whether motoneurons react to signals deriving from target inflammation, we studied the facial motor nucleus after injections of phytohaemagglutinin in the snout of adult rats. This plant lectin is a tool widely used to induce proliferation and activation of T lymphocytes, and we observed marked lymphocyte infiltration in the injected facial muscles. Retrograde labelling of motoneurons was not detected after peripheral injections of fluorochrome-conjugated phytohaemagglutinin. Nitric oxide synthase, revealed by NADPH-diaphorase histochemistry, OX-42-immunoreactive microglia, and expression of the cell death repressor gene bcl-2, investigated with nonradioactive in situ hybridization and immunohistochemistry, were evaluated in the facial nucleus. Daily phytohaemagglutinin injections for 4 days, mimicking repeated muscle exposure to inflammatory stimuli, resulted after 2-day survival in NADPH-diaphorase induction in motoneurons and marked activation of the surrounding microglia. Quantitative image analysis of NADPH-diaphorase staining, and OX-42 immunoreactivity and microglial cell counts indicated highly significant increases with respect to saline-injected control cases. The occurrence of a neuroprotective retrograde response was evaluated monitoring bcl-2 expression. Following single phytohaemagglutinin administration, bcl-2 mRNA was significantly upregulated at 6 h in facial motoneurons and returned to basal levels at 24 h. Bcl-2 immunoreactivity was markedly upregulated at 24 h and was still significantly higher than in controls at 7 days, when concomitant NADPH-diaphorase induction in motoneurons and microglia activation was also observed. No degenerative features were observed in motoneurons after phytohaemagglutinin injections at the examined time-points. The data point out that local muscle inflammation retrogradely elicits gene activation in motoneurons and their microenvironment.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD147,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Avian Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bsg protein, Gallus gallus,
http://linkedlifedata.com/resource/pubmed/chemical/Bsg protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0953-816X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
13
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1329-38
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11298793-Animals,
pubmed-meshheading:11298793-Antigens, CD,
pubmed-meshheading:11298793-Antigens, CD147,
pubmed-meshheading:11298793-Antigens, Neoplasm,
pubmed-meshheading:11298793-Antigens, Surface,
pubmed-meshheading:11298793-Avian Proteins,
pubmed-meshheading:11298793-Blood Proteins,
pubmed-meshheading:11298793-Facial Nerve,
pubmed-meshheading:11298793-Gene Expression,
pubmed-meshheading:11298793-Lymphocytes,
pubmed-meshheading:11298793-Male,
pubmed-meshheading:11298793-Membrane Glycoproteins,
pubmed-meshheading:11298793-Microglia,
pubmed-meshheading:11298793-Motor Neurons,
pubmed-meshheading:11298793-Muscle, Skeletal,
pubmed-meshheading:11298793-Myositis,
pubmed-meshheading:11298793-NADPH Dehydrogenase,
pubmed-meshheading:11298793-Nitric Oxide Synthase,
pubmed-meshheading:11298793-Phytohemagglutinins,
pubmed-meshheading:11298793-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11298793-RNA, Messenger,
pubmed-meshheading:11298793-Rats,
pubmed-meshheading:11298793-Rats, Wistar
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| pubmed:year |
2001
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| pubmed:articleTitle |
Retrograde response of the rat facial motor nucleus to muscle inflammation elicited by phytohaemagglutinin.
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| pubmed:affiliation |
Department of Morphological and Biomedical Sciences, Section of Anatomy and Histology, Medical Faculty, Strada Le Grazie 8, 37134 Verona, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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