Linked Life Data

11298336

Source:http://linkedlifedata.com/resource/pubmed/id/11298336

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-4-12
pubmed:abstractText
Therapeutic intervention with antiretroviral therapy (ART) enables the modulation of HIV virus load and hence provides a unique opportunity to study the consequences of varying antigen load on the phenotype of virus-specific CD8(+) T lymphocytes in a persistent human viral infection. The recent advent of tetrameric peptide / HLA class I complexes has enabled the direct phenotypic characterization of antigen-specific T cell populations ex vivo. Here, we use this technology to examine directly ex vivo the consequences of therapeutic manipulation of HIV virus load on the phenotype of HIV-specific CTL. Our observations show that: (1) distinct sequential activation patterns of CD8(+) T cells are associated with increasing virus load; (2) T cell receptor (TCR) down-regulation without apoptosis represents an early event during the generation of a T cell response in a natural infection and precedes the emergence of two distinct antigen-specific CD8(+) T cell populations which differ in TCR and CD8 expression levels. Clear differences in surface Annexin V staining were observed between these populations. The observation that CTL activation, demonstrated by TCR and CD8 down-regulation, in response to rising levels of virus load, co-segregates with apoptosis only during later stages of the response indicates that antigen-associated cell death is restricted to distinct subpopulations of CTL.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1115-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11298336-Annexin A5, pubmed-meshheading:11298336-Anti-HIV Agents, pubmed-meshheading:11298336-Antigens, CD8, pubmed-meshheading:11298336-Apoptosis, pubmed-meshheading:11298336-CD8-Positive T-Lymphocytes, pubmed-meshheading:11298336-Cells, Cultured, pubmed-meshheading:11298336-Chronic Disease, pubmed-meshheading:11298336-Down-Regulation, pubmed-meshheading:11298336-Flow Cytometry, pubmed-meshheading:11298336-HIV Infections, pubmed-meshheading:11298336-HIV-1, pubmed-meshheading:11298336-Histocompatibility Antigens Class I, pubmed-meshheading:11298336-Humans, pubmed-meshheading:11298336-Immunophenotyping, pubmed-meshheading:11298336-Lymphocyte Activation, pubmed-meshheading:11298336-Peptides, pubmed-meshheading:11298336-Receptors, Antigen, T-Cell, pubmed-meshheading:11298336-Viral Load
pubmed:year
2001
pubmed:articleTitle
Direct ex vivo analysis reveals distinct phenotypic patterns of HIV-specific CD8(+) T lymphocyte activation in response to therapeutic manipulation of virus load.
pubmed:affiliation
Nuffield Department of Medicine, John Radcliffe Hospital, Oxford, Great Britain. annette.oxenius@ndm.ox.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't