Source:http://linkedlifedata.com/resource/pubmed/id/11292181
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2001-4-6
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pubmed:abstractText |
The effect of histamine on intracellular free Ca2+ levels ([Ca2+]i) in HA22/VGH human hepatoma cells were evaluated using fura-2 as a fluorescent Ca2+ dye. Histamine (0.2-5 microM) increased [Ca2+]i in a concentration-dependent manner with an EC50 value of about 1 microM. The [Ca2+]i response comprised an initial rise, a slow decay, and a sustained phase. Extracellular Ca2+ removal inhibited 50% of the [Ca2+]i signal. In Ca2+-free medium, after cells were treated with 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor), 5 microM histamine failed to increase [Ca2+]i. After pretreatment with 5 microM histamine in Ca2+-free medium for 4 min, addition of 3 mM Ca2+ induced a [Ca2+]i increase of a magnitude 7-fold greater than control. Histamine (5 microM)-induced intracellular Ca2+ release was abolished by inhibiting phospholipase C with 2 microM 1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione (U73122), and by 5 microM pyrilamine but was not altered by 50 microM cimetidine. Together, this study shows that histamine induced [Ca2+]i increases in human hepatoma cells by stimulating H1, but not H2, histamine receptors. The [Ca2+]i signal was caused by Ca2+ release from thapsigargin-sensitive endoplasmic reticulum in an inositol 1,4,5-trisphosphate-dependent manner, accompanied by Ca2+ entry.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cimetidine,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine H1 Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine H2 Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrilamine
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0304-4920
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
165-9
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pubmed:dateRevised |
2009-8-12
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pubmed:meshHeading |
pubmed-meshheading:11292181-Biological Transport,
pubmed-meshheading:11292181-Calcium,
pubmed-meshheading:11292181-Carcinoma, Hepatocellular,
pubmed-meshheading:11292181-Cimetidine,
pubmed-meshheading:11292181-Cytosol,
pubmed-meshheading:11292181-Extracellular Space,
pubmed-meshheading:11292181-Fluorescent Dyes,
pubmed-meshheading:11292181-Fura-2,
pubmed-meshheading:11292181-Histamine,
pubmed-meshheading:11292181-Histamine H1 Antagonists,
pubmed-meshheading:11292181-Histamine H2 Antagonists,
pubmed-meshheading:11292181-Humans,
pubmed-meshheading:11292181-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:11292181-Liver Neoplasms,
pubmed-meshheading:11292181-Pyrilamine,
pubmed-meshheading:11292181-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Histamine-Induced increases in intracellular free Ca2+ levels in hepatoma cells.
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pubmed:affiliation |
Department of Internal Medicine, Kaohsiung Veterans General Hospital, Taiwan, ROC.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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