Source:http://linkedlifedata.com/resource/pubmed/id/11249876
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2001-3-16
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| pubmed:abstractText |
We investigated whether activation of A(1) or A(3) adenosine receptors (ARs) induces late preconditioning (PC) against infarction in conscious rabbits using the selective AR agonists 2-chloro-N(6)-cyclopentyladenosine (CCPA) and N(6)-3-iodobenzyladenosine-5'-N-methylcarboxamide (IB-MECA). In vitro radioligand binding and cAMP assays demonstrated CCPA to be approximately 200- to 400-fold selective for the rabbit A(1)AR and IB-MECA to be approximately 20-fold selective for the rabbit A(3)AR. We observed that (1) pretreatment of rabbits 24 hours earlier with CCPA (100 microgram/kg IV bolus) or IB-MECA (100 or 300 microgram/kg) resulted in an approximately 35% to 40% reduction in the size of the infarct induced by 30 minutes of coronary artery occlusion and 72 hours of reperfusion compared with vehicle-treated rabbits, whereas pretreatment with the selective A(2A)AR agonist CGS 21680 (100 microgram/kg) had no effect; (2) the delayed cardioprotective effect of CCPA, but not that of IB-MECA, was completely blocked by coadministration of the highly selective A(1)AR antagonist N-0861; (3) inhibition of nitric oxide synthase (NOS) with N(omega)-nitro-L-arginine during the 30-minute occlusion abrogated the infarct-sparing action of CCPA but not that of IB-MECA; and (4) inhibition of ATP-sensitive potassium (K(ATP)) channels with sodium 5-hydroxydecanoate during the 30-minute occlusion blocked the cardioprotective effects of both CCPA and IB-MECA. Taken together, these results indicate that activation of either A(1)ARs or A(3)ARs (but not A(2A)ARs) elicits delayed protection against infarction in conscious rabbits and that both A(1)AR- and A(3)AR-induced cardioprotection involves opening of K(ATP) channels. However, A(1)AR-induced late PC uses an NOS-dependent pathway whereas A(3)AR-induced late PC is mediated by an NOS-independent pathway.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-(2-carboxyethyl)phenethylamino)...,
http://linkedlifedata.com/resource/pubmed/chemical/2-chloro-N(6)cyclopentyladenosine,
http://linkedlifedata.com/resource/pubmed/chemical/5-hydroxydecanoic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Decanoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxy Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/N(6)-(3-iodobenzyl)-5'-N-methylcarbo...,
http://linkedlifedata.com/resource/pubmed/chemical/N 0861,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroarginine,
http://linkedlifedata.com/resource/pubmed/chemical/Norbornanes,
http://linkedlifedata.com/resource/pubmed/chemical/Phenethylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Adenosine A3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1524-4571
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
16
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| pubmed:volume |
88
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
520-8
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11249876-Adenine,
pubmed-meshheading:11249876-Adenosine,
pubmed-meshheading:11249876-Animals,
pubmed-meshheading:11249876-Binding, Competitive,
pubmed-meshheading:11249876-Cell Line,
pubmed-meshheading:11249876-Consciousness,
pubmed-meshheading:11249876-Decanoic Acids,
pubmed-meshheading:11249876-Humans,
pubmed-meshheading:11249876-Hydroxy Acids,
pubmed-meshheading:11249876-Iodine Radioisotopes,
pubmed-meshheading:11249876-Ischemic Preconditioning, Myocardial,
pubmed-meshheading:11249876-Membranes,
pubmed-meshheading:11249876-Myocardial Infarction,
pubmed-meshheading:11249876-Nitroarginine,
pubmed-meshheading:11249876-Norbornanes,
pubmed-meshheading:11249876-Phenethylamines,
pubmed-meshheading:11249876-Rabbits,
pubmed-meshheading:11249876-Radioligand Assay,
pubmed-meshheading:11249876-Receptor, Adenosine A3,
pubmed-meshheading:11249876-Receptors, Purinergic P1
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| pubmed:year |
2001
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| pubmed:articleTitle |
A(1) or A(3) adenosine receptors induce late preconditioning against infarction in conscious rabbits by different mechanisms.
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| pubmed:affiliation |
Division of Cardiology, University of Louisville and Jewish Heart and Lung Institute, Louisville, KY, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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