Source:http://linkedlifedata.com/resource/pubmed/id/11248212
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-3-15
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| pubmed:abstractText |
The roles of serum mannan binding protein (MBP) and the mannose receptor in the cellular uptake of mannosylated liposomes (Man-liposomes) by macrophages were studied. Man-liposomes were prepared by incorporating cholesten-5-yloxy-N-(4-((1-imino-2-beta-D-thiomannosylethyl)amino)butyl)formamide (Man-C4-Chol) into small unilamellar long circulating liposomes consisting of cholesterol (Chol) and distearoyl phosphatidylcholine (DSPC). In the in vitro cellular uptake study with cultured mouse peritoneal macrophages, [(3)H]Man-liposomes were taken up to a great extent, whereas no significant uptake was observed for [(3)H]cholesterol and DSPC liposomes without Man-C4-Chol (Bare-liposomes). The uptake of [(3)H]Man-liposomes was dose- and temperature-dependent and inhibited by an excess of mannosylated bovine serum albumin, suggesting their specific uptake via membrane mannose receptor-mediated endocytosis. Furthermore, it was demonstrated that (111)In-MBP binds strongly to Man-liposomes based on the recognition of Man-C4-Chol and markedly enhanced their uptake by macrophages. These results are supported by confocal laser microscopic images. In addition, in vivo hepatic uptake of (111)In-MBP was enhanced by Man-liposomes. On the other hand, the uptake of Man-liposomes was significantly reduced by preincubation with serum and further with MBP-depleted serum suggesting inhibitory effects of serum proteins such as albumin on mannose receptor-mediated endocytosis. The involvement of serum-type MBP and membrane mannose receptors in the uptake of Man-liposomes is thus suggested.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collectins,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Indium Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose-Binding Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin,
http://linkedlifedata.com/resource/pubmed/chemical/mannose receptor,
http://linkedlifedata.com/resource/pubmed/chemical/mannose-bovine serum albumin...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0006-3002
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
9
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| pubmed:volume |
1511
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
134-45
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11248212-Animals,
pubmed-meshheading:11248212-Carrier Proteins,
pubmed-meshheading:11248212-Cells, Cultured,
pubmed-meshheading:11248212-Collectins,
pubmed-meshheading:11248212-Drug Carriers,
pubmed-meshheading:11248212-Indium Radioisotopes,
pubmed-meshheading:11248212-Lectins, C-Type,
pubmed-meshheading:11248212-Liposomes,
pubmed-meshheading:11248212-Liver,
pubmed-meshheading:11248212-Macrophages,
pubmed-meshheading:11248212-Male,
pubmed-meshheading:11248212-Mannose,
pubmed-meshheading:11248212-Mannose-Binding Lectins,
pubmed-meshheading:11248212-Mice,
pubmed-meshheading:11248212-Mice, Inbred ICR,
pubmed-meshheading:11248212-Microscopy, Confocal,
pubmed-meshheading:11248212-Receptors, Cell Surface,
pubmed-meshheading:11248212-Serum Albumin
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| pubmed:year |
2001
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| pubmed:articleTitle |
Involvement of serum mannan binding proteins and mannose receptors in uptake of mannosylated liposomes by macrophages.
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| pubmed:affiliation |
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
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| pubmed:publicationType |
Journal Article
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