| pubmed-article:11228378 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0020971 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0074448 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0008356 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0028429 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0475463 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C1518413 | lld:lifeskim |
| pubmed-article:11228378 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:11228378 | pubmed:issue | 15-16 | lld:pubmed |
| pubmed-article:11228378 | pubmed:dateCreated | 2001-3-6 | lld:pubmed |
| pubmed-article:11228378 | pubmed:abstractText | Escherichia coli O157:H7 produces two forms of verotoxin (VT), VT1 and VT2, which cause hemorrhagic colitis with development, in some cases, of hemolytic uremic syndrome. These toxins consist of an enzymatically active A subunit and pentamers of B subunit responsible for their binding to host cells. We used the secretion-expression system of Bacillus brevis to produce recombinant VT1B and VT2B. The secreted B subunits were purified and sequenced to verify their structure. Receptor-binding showed that rVT1B but not rVT2B bound to Gb3-receptor. When mice were nasally immunized with rVT1B or rVT2B together with a nontoxic mutant of cholera toxin (mCT) or native cholera toxin (nCT) as adjuvants, serum IgG and mucosal IgA antibody responses to VT1B were induced. The VT1B-specific antibodies prevented VT1B binding to its Gb3 receptor. In contrast, poor serum and no mucosal VT2B-specific antibodies but brisk CTB-specific antibody responses were induced by nasal immunization with rVT2B in the presence of mCT or nCT. These results show that nasal immunization with rVTB and mCT as a nontoxic mucosal adjuvant is an effective regimen for the induction of VT1B but not VT2B antibody responses which inhibit VT1B binding to Gb3 receptor. | lld:pubmed |
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| pubmed-article:11228378 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11228378 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11228378 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11228378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11228378 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11228378 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11228378 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11228378 | pubmed:issn | 0264-410X | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:YamamotoSS | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:TakedaYY | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:McGheeJ RJR | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:KiyonoHH | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:KohsakaTT | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:YukiYY | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:FujihashiKK | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:UdakaSS | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:OhmuraMM | lld:pubmed |
| pubmed-article:11228378 | pubmed:author | pubmed-author:ByunYY | lld:pubmed |
| pubmed-article:11228378 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11228378 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:11228378 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:11228378 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11228378 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11228378 | pubmed:pagination | 2061-70 | lld:pubmed |
| pubmed-article:11228378 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:11228378 | pubmed:meshHeading | pubmed-meshheading:11228378... | lld:pubmed |
| pubmed-article:11228378 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11228378 | pubmed:articleTitle | Nasal immunization with E. coli verotoxin 1 (VT1)-B subunit and a nontoxic mutant of cholera toxin elicits serum neutralizing antibodies. | lld:pubmed |
| pubmed-article:11228378 | pubmed:affiliation | JCR Biopharmaceuticals Incorporated, , San Diego, CA 92121-1194, USA. | lld:pubmed |
| pubmed-article:11228378 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11228378 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11228378 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11228378 | lld:pubmed |
