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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-3-14
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pubmed:abstractText |
IgAN is a common form of primary glomerulonephritis and also a disease of tonsillar focal infection. The comprehensive mechanism underlying this disease remains to be defined. To better understand its pathogenesis, we investigated tonsillar CD5+ B cells (B-1 cells) with respect to IgA synthesis. Germinal centre (GC) B cells were isolated from the tonsils of IgAN patients and the number of B-1 cells in the GC determined by flow cytometry. GC B-1 and B-2 (CD5- B) cells were purified by cell sorter, the cells were incubated with agonist anti-CD40 MoAb and the ability for antibody production by B-1 and B-2 cells determined by ELISPOT assay. GC B-1 cells and B-2 cells were incubated with agonist anti-Fas MoAb, and apoptosis in GC B-1 cells and B-2 cells was analysed by flow cytometry. Although B-1 cells do not usually take part in the GC reaction, an increase in B-1 cell numbers was observed in the GC of tonsils from IgAN patients. These B-1 cells were likely IgA1 antibody-producing cells, since the prominent IgA subclass in IgAN is generally considered to be IgA1. Although Fas-dependent apoptosis is essential for the elimination of activated B cells, these B-1 cells showed a reduced susceptibility to Fas-mediated apoptosis. It is conceivable that activated B-1 cells may survive in the GC due to impaired apoptosis and thus produce abnormal antibodies. These findings suggest that the immune responses of B-1 cells in the tonsillar GC could thus have an impact on the pathogenesis of IgAN.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11207662-1375924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11207662-1376087,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
301-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11207662-Adolescent,
pubmed-meshheading:11207662-Adult,
pubmed-meshheading:11207662-Antigens, CD95,
pubmed-meshheading:11207662-Apoptosis,
pubmed-meshheading:11207662-Cell Count,
pubmed-meshheading:11207662-Female,
pubmed-meshheading:11207662-Germinal Center,
pubmed-meshheading:11207662-Glomerulonephritis, IGA,
pubmed-meshheading:11207662-Humans,
pubmed-meshheading:11207662-Male,
pubmed-meshheading:11207662-Middle Aged,
pubmed-meshheading:11207662-Palatine Tonsil
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pubmed:year |
2001
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pubmed:articleTitle |
Increase in tonsillar germinal centre B-1 cell numbers in IgA nephropathy (IgAN) patients and reduced susceptibility to Fas-mediated apoptosis.
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pubmed:affiliation |
Department of Otolaryngology, Oita Medical University, Hazama-machi, Oita, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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