Source:http://linkedlifedata.com/resource/pubmed/id/11202156
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2001-1-29
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| pubmed:abstractText |
P-glycoprotein is a plasma membrane efflux pump which is responsible for multidrug resistance of many cancer cell lines. A number of studies have demonstrated the presence of P-glycoprotein molecules, besides on the plasma membrane, also in intracellular sites, such as the Golgi apparatus and the nucleus. In this study, the presence and function of P-glycoprotein in the nuclear membranes of human breast cancer cells (MCF-7 WT) and their multidrug resistant variants (MCF-7 DX) were investigated. Electron and confocal microscopy immunolabelling experiments demonstrated the presence of P-glycoprotein molecules in the nuclear membranes of MCF-7 DX cells. Moreover, the labelling pattern was strongly dependent on pH values of the incubation buffer. At physiological pH (7.2), a strong labelling was detected in the cytoplasm and the nuclear matrix in both sensitive and resistant MCF-7 cells. By raising the pH to 8.0, the P-glycoprotein molecules were easily detected in the cytoplasm (transport vesicles and Golgi apparatus), plasma and nuclear membranes exclusively in MCF-7 DX cells. Furthermore, drug uptake and efflux studies, performed by flow cytometry on isolated nuclei in the presence of the P-glycoprotein inhibitor cyclosporin A, suggested the presence of a functional P-glycoprotein in the nuclear membrane, but not in the nuclear matrix, of drug resistant cells. Therefore, P-glycoprotein in the nuclear envelope seems to represent a further defense mechanism developed by resistant cells against antineoplastic agents.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0018-2214
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
32
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
599-606
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11202156-Antineoplastic Agents,
pubmed-meshheading:11202156-DNA, Neoplasm,
pubmed-meshheading:11202156-Doxorubicin,
pubmed-meshheading:11202156-Drug Resistance, Multiple,
pubmed-meshheading:11202156-Female,
pubmed-meshheading:11202156-Flow Cytometry,
pubmed-meshheading:11202156-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:11202156-Humans,
pubmed-meshheading:11202156-Microscopy, Confocal,
pubmed-meshheading:11202156-Microscopy, Immunoelectron,
pubmed-meshheading:11202156-Nuclear Envelope,
pubmed-meshheading:11202156-P-Glycoprotein,
pubmed-meshheading:11202156-Tumor Cells, Cultured
|
| pubmed:year |
2000
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| pubmed:articleTitle |
Detection of P-glycoprotein in the nuclear envelope of multidrug resistant cells.
|
| pubmed:affiliation |
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
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| pubmed:publicationType |
Journal Article
|