Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-2-22
pubmed:abstractText
The budding of clathrin-coated vesicles is essential for protein transport. After budding, clathrin must be uncoated before the vesicles can fuse with other membranous structures. In vitro, the molecular chaperone Hsc70 uncoats clathrin-coated vesicles in an ATP-dependent process that requires a specific J-domain protein such as auxilin. However, there is little evidence that either Hsc70 or auxilin is essential in vivo. Here we show that C. elegans has a single auxilin homologue that is identical to mammalian auxilin in its in vitro activity. When RNA-mediated interference (RNAi) is used to inhibit auxilin expression in C. elegans, oocytes show markedly reduced receptor-mediated endocytosis of yolk protein tagged with green fluorescent protein (GFP). In addition, most of these worms arrest during larval development, exhibit defective distribution of GFP-clathrin in many cell types, and show a marked change in clathrin dynamics, as determined by fluorescence recovery after photobleaching (FRAP). We conclude that auxilin is required for in vivo clathrin-mediated endocytosis and development in C. elegans.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular..., http://linkedlifedata.com/resource/pubmed/chemical/Clathrin, http://linkedlifedata.com/resource/pubmed/chemical/Egg Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents, http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1465-7392
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-9
pubmed:dateRevised
2011-9-22
pubmed:meshHeading
pubmed-meshheading:11175756-Adaptor Proteins, Vesicular Transport, pubmed-meshheading:11175756-Animals, pubmed-meshheading:11175756-Animals, Genetically Modified, pubmed-meshheading:11175756-Caenorhabditis elegans, pubmed-meshheading:11175756-Clathrin, pubmed-meshheading:11175756-Clathrin-Coated Vesicles, pubmed-meshheading:11175756-Egg Proteins, pubmed-meshheading:11175756-Endocytosis, pubmed-meshheading:11175756-Enzyme Inhibitors, pubmed-meshheading:11175756-Green Fluorescent Proteins, pubmed-meshheading:11175756-Indicators and Reagents, pubmed-meshheading:11175756-Luminescent Proteins, pubmed-meshheading:11175756-Microscopy, Fluorescence, pubmed-meshheading:11175756-Molecular Chaperones, pubmed-meshheading:11175756-Nerve Tissue Proteins, pubmed-meshheading:11175756-Oocytes, pubmed-meshheading:11175756-Phosphoproteins, pubmed-meshheading:11175756-RNA, pubmed-meshheading:11175756-Recombinant Fusion Proteins
pubmed:year
2001
pubmed:articleTitle
Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo.
pubmed:affiliation
Laboratory of Cell Biology, NHLBI, NIH, Bethesda, Md 20892-0301, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't