Linked Life Data

11172048

Source:http://linkedlifedata.com/resource/pubmed/id/11172048

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-2-26
pubmed:abstractText
The worldwide threat of tuberculosis to human health emphasizes the need to develop novel approaches to a global epidemiological surveillance. The current standard for Mycobacterium tuberculosis typing based on IS6110 restriction fragment length polymorphism (RFLP) suffers from the difficulty of comparing data between independent laboratories. Here, we propose a high-resolution typing method based on variable number tandem repeats (VNTRs) of genetic elements named mycobacterial interspersed repetitive units (MIRUs) in 12 human minisatellite-like regions of the M. tuberculosis genome. MIRU-VNTR profiles of 72 different M. tuberculosis isolates were established by PCR analysis of all 12 loci. From 2 to 8 MIRU-VNTR alleles were identified in the 12 regions in these strains, which corresponds to a potential of over 16 million different combinations, yielding a resolution power close to that of IS6110-RFLP. All epidemiologically related isolates tested were perfectly clustered by MIRU-VNTR typing, indicating that the stability of these MIRU-VNTRs is adequate to track outbreak episodes. The correlation between genetic relationships inferred from MIRU-VNTR and IS6110-RFLP typing was highly significant. Compared with IS6110-RFLP, high-resolution MIRU-VNTR typing has the considerable advantages of being fast, appropriate for all M. tuberculosis isolates, including strains that have a few IS6110 copies, and permitting easy and rapid comparison of results from independent laboratories. This typing method opens the way to the construction of digital global databases for molecular epidemiology studies of M. tuberculosis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10203468, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10220886, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10383858, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10405410, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10438361, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10459922, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10459924, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10479153, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10479154, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10589725, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10612319, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10690415, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-10844663, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-1509256, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-1961248, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-2320563, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-2425735, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-6018555, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-7757798, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-7907344, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-7910661, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-8008034, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-8586708, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-8627966, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-8727873, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9003621, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9157152, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9275218, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9276389, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9378125, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9393791, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9426136, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9501229, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9534994, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9542912, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9555892, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9574716, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9611793, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9618442, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9692179, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9705376, http://linkedlifedata.com/resource/pubmed/commentcorrection/11172048-9986855
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1901-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology.
pubmed:affiliation
Laboratoire de Recherche et Développement, Institut National de la Santé et de la Recherche Médicale, U447, Institut Pasteur de Lille, 1, Rue du Prof. Calmette, F-59019 Lille Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't