Linked Life Data

11166656

Source:http://linkedlifedata.com/resource/pubmed/id/11166656

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-2-22
pubmed:abstractText
Background: in response to a variety of stimuli, phagocytes release large quantities of reactive oxygen species (ROS), which are essential for bacterial killing. However, excessive ROS production not appropriately compensated by antioxidant molecules can lead to oxidative stress, which may also play an important role in pathogenesis of HIV infection. In fact, ROS participate in chronic inflammation, HIV replication and the apoptosis of cells of the immune system. Objective and study design: we used flow cytometry to study, in whole blood, the activation and redox status of polymorphonuclear neutrophils (PMN) and monocytes at different stages of the disease. Results: we showed that neutrophils and monocytes from HIV-infected patients spontaneously produced increased amounts of H2O2. This increased H2O2 production was associated with alterations of adhesion molecules expression at the cell surface, which also reflected basal activation of phagocytes from the HIV-infected patients. In monocytes, basal H2O2 production correlated with viral load. This increased ROS production was associated with changes in the expression of the antiapoptotic/antioxidant compounds Bcl-2 and thioredoxin along the course of the disease. This modulation could result from a dual regulation by oxidative stress and could explain at least in part why monocyte numbers remain relatively stable throughout the disease. Monocytes expressed a normal maximal capacity to produce ROS in optimal conditions of stimulation. In contrast, after ex vivo priming with TNFalpha or IL-8, neutrophils showed a decreased H2O2 production in response to bacterial N-formyl peptides. This latter impairment correlated with the decrease in CD4+ lymphocyte numbers and with IL-8 and IL-6 plasma levels. Conclusions: the increased basal ROS production by phagocytes could participate to the oxidative injury which has been implicated in the pathophysiology of HIV infection. In addition, the decreased priming of H2O2 production by neutrophils could contribute to the increased susceptibility of HIV-infected patients to bacterial infections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1386-6532
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-109
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11166656-Actins, pubmed-meshheading:11166656-Adult, pubmed-meshheading:11166656-Antigens, CD14, pubmed-meshheading:11166656-Cytokines, pubmed-meshheading:11166656-Flow Cytometry, pubmed-meshheading:11166656-HIV Seropositivity, pubmed-meshheading:11166656-HIV-1, pubmed-meshheading:11166656-Humans, pubmed-meshheading:11166656-Hydrogen Peroxide, pubmed-meshheading:11166656-L-Selectin, pubmed-meshheading:11166656-Macrophage-1 Antigen, pubmed-meshheading:11166656-Monocytes, pubmed-meshheading:11166656-Neutrophil Activation, pubmed-meshheading:11166656-Neutrophils, pubmed-meshheading:11166656-Oxidation-Reduction, pubmed-meshheading:11166656-Oxidative Stress, pubmed-meshheading:11166656-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:11166656-Reactive Oxygen Species, pubmed-meshheading:11166656-Thioredoxins
pubmed:year
2001
pubmed:articleTitle
The role of phagocytes in HIV-related oxidative stress.
pubmed:affiliation
INSERM U 479 and Service d'Immunologie et d'Hématologie, CHU X. Bichat, 46 rue Henri Huchard, 75877 Paris Cedex, France.
pubmed:publicationType
Journal Article