|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-2-22
|
|
pubmed:abstractText |
The role of K396 in the enzymatic catalysis and the antigenicity of the 65 kDa isoform of glutamate decarboxylase (GAD65) was analyzed using the K396R GAD65 mutant. GAD65 is a major autoantigen in Type 1 diabetes and autoantibodies directed to GAD65 are widely used markers for this disease. We found that (1) recombinant human GAD65 is fully enzymatically active; (2) the K396R mutation abolished GAD65 activity; and (3) the K396R mutant retained full antigenicity to GAD65 autoantibodies in serum from Type 1 diabetes patients, but not to polyclonal antibodies raised to the catalytic domain.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
19
|
|
pubmed:volume |
488
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
185-9
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:11163769-Amino Acid Sequence,
pubmed-meshheading:11163769-Amino Acid Substitution,
pubmed-meshheading:11163769-Animals,
pubmed-meshheading:11163769-Autoantibodies,
pubmed-meshheading:11163769-Autoantigens,
pubmed-meshheading:11163769-Binding Sites,
pubmed-meshheading:11163769-Catalysis,
pubmed-meshheading:11163769-Catalytic Domain,
pubmed-meshheading:11163769-Diabetes Mellitus, Type 1,
pubmed-meshheading:11163769-Glutamate Decarboxylase,
pubmed-meshheading:11163769-Humans,
pubmed-meshheading:11163769-Hydrogen-Ion Concentration,
pubmed-meshheading:11163769-Immune Sera,
pubmed-meshheading:11163769-Isoenzymes,
pubmed-meshheading:11163769-Kinetics,
pubmed-meshheading:11163769-Molecular Sequence Data,
pubmed-meshheading:11163769-Molecular Weight,
pubmed-meshheading:11163769-Mutagenesis, Site-Directed,
pubmed-meshheading:11163769-Mutation,
pubmed-meshheading:11163769-Pichia,
pubmed-meshheading:11163769-Pyridoxal Phosphate,
pubmed-meshheading:11163769-Radioimmunoassay,
pubmed-meshheading:11163769-Recombinant Proteins,
pubmed-meshheading:11163769-Reducing Agents,
pubmed-meshheading:11163769-Transformation, Genetic
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Site-directed mutagenesis of K396R of the 65 kDa glutamic acid decarboxylase active site obliterates enzyme activity but not antibody binding.
|
|
pubmed:affiliation |
Department of Medicine, University of Washington, Seattle 98195, USA. champe@u.washington.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
