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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
Integrins are highly regulated receptors that can function in both cell-substrate and cell-cell adhesion. We have found that the activating anti-beta1 mAb, 12G10, can specifically and rapidly induce both cell-substrate and cell-cell adhesion of HT-1080 human fibrosarcoma and other cell types. Binding of mAb 12G10 induced clustering of cell-surface integrins, and the preferential localization of beta1 integrins expressing the 12G10 epitope at cell-cell adhesion sites. Fab fragments of mAb 12G10 induced HT-1080 cell-cell adhesion as effectively as did intact antibodies, suggesting that integrin clustering was not due to direct antibody crosslinking. Latrunculin B, an inhibitor of F-actin polymerization, inhibited cell-cell adhesion but not the clustering of integrins. Results from a novel, two-color cell-cell adhesion assay suggested that nonactivated cells can bind to activated cells and that integrin activation-induced HT-1080 cell-cell adhesion minimally requires the interaction of activated alpha2beta1 with nonactivated alpha3beta1. These findings suggest that HT-1080 cell-cell adhesion induced by integrin activation require a signaling process involving integrin clustering and the subsequent organization of the cytoskeleton. Integrin activation could therefore play a key role in cell-cell adhesion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29,
http://linkedlifedata.com/resource/pubmed/chemical/Bicyclo Compounds, Heterocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Cations, Divalent,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidines,
http://linkedlifedata.com/resource/pubmed/chemical/latrunculin B
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-4827
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
65-76
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11161706-Actins,
pubmed-meshheading:11161706-Antibodies, Monoclonal,
pubmed-meshheading:11161706-Antigens, CD29,
pubmed-meshheading:11161706-Bicyclo Compounds, Heterocyclic,
pubmed-meshheading:11161706-Cadherins,
pubmed-meshheading:11161706-Cations, Divalent,
pubmed-meshheading:11161706-Cell Adhesion,
pubmed-meshheading:11161706-Collagen,
pubmed-meshheading:11161706-Dose-Response Relationship, Immunologic,
pubmed-meshheading:11161706-Epitopes,
pubmed-meshheading:11161706-Fibronectins,
pubmed-meshheading:11161706-Fibrosarcoma,
pubmed-meshheading:11161706-Humans,
pubmed-meshheading:11161706-Hybridomas,
pubmed-meshheading:11161706-Immunohistochemistry,
pubmed-meshheading:11161706-Laminin,
pubmed-meshheading:11161706-Microscopy, Confocal,
pubmed-meshheading:11161706-Models, Biological,
pubmed-meshheading:11161706-Signal Transduction,
pubmed-meshheading:11161706-Thiazoles,
pubmed-meshheading:11161706-Thiazolidines,
pubmed-meshheading:11161706-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
Activation of beta1 integrins induces cell-cell adhesion.
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pubmed:affiliation |
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
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pubmed:publicationType |
Journal Article
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