| pubmed-article:11152647 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0040132 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C1442757 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C1824584 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11152647 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:11152647 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11152647 | pubmed:dateCreated | 2001-1-26 | lld:pubmed |
| pubmed-article:11152647 | pubmed:abstractText | Surfactant protein (SP)-B expression is restricted to type II pneumocytes and Clara cells in the lung. Previously, a promoter region of human SP-B gene from -64 to -118 has been identified as critical for the tissue-specific expression of this gene. Two cis-elements for thyroid transcription factor (TTF)-1 and hepatocyte nuclear factor (HNF)-3alpha binding were found within this area. Using an oligonucleotide fragment, we incorporated this region sequence into the promoter of a HIS3 reporter gene in yeast. With this modified yeast a human lung complementary DNA (cDNA) library was screened for DNA-binding proteins, other than TTF-1 and HNF-3alpha, that interacted with this promoter segment. A cDNA clone encoding a novel polypeptide, BR22, was identified that activated the reporter gene expression in yeast. This gene is expressed in many tissues and encodes a protein with bipartite nuclear localization signals. Studies using in vivo yeast two-hybrid analysis, in vitro protein-protein interactions, and coimmunoprecipitation analyses demonstrated that BR22 formed a protein complex with TTF-1. In vivo cotransfection studies further indicated that BR22 could act with TTF-1 to synergistically activate the SP-B promoter in mammalian cells. Our data suggest that BR22 is a TTF-1-associated protein. Through a protein-protein interaction with TTF-1, BR22 can form a complex and activate the human SP-B promoter in vivo. | lld:pubmed |
| pubmed-article:11152647 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11152647 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11152647 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11152647 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11152647 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11152647 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11152647 | pubmed:issn | 1044-1549 | lld:pubmed |
| pubmed-article:11152647 | pubmed:author | pubmed-author:YangM CMC | lld:pubmed |
| pubmed-article:11152647 | pubmed:author | pubmed-author:WandAA | lld:pubmed |
| pubmed-article:11152647 | pubmed:author | pubmed-author:YangY SYS | lld:pubmed |
| pubmed-article:11152647 | pubmed:author | pubmed-author:WeisslerJ CJC | lld:pubmed |
| pubmed-article:11152647 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11152647 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:11152647 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11152647 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11152647 | pubmed:pagination | 30-37 | lld:pubmed |
| pubmed-article:11152647 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:11152647 | pubmed:meshHeading | pubmed-meshheading:11152647... | lld:pubmed |
| pubmed-article:11152647 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11152647 | pubmed:articleTitle | BR22, a novel protein, interacts with thyroid transcription factor-1 and activates the human surfactant protein B promoter. | lld:pubmed |
| pubmed-article:11152647 | pubmed:affiliation | Department of Internal Medicine, Pulmonary and Critical Care Medicine, UT Southwestern Medical Center, Dallas, Texas 75390-9034, USA. Yih-Shing.Yang@UTSouthwestern.edu. | lld:pubmed |
| pubmed-article:11152647 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11152647 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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